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Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa punctata)

Year 2011, Volume: 11 Issue: 3, - , 01.06.2011

Abstract

To characterize whether environmental low temperature is involved in metabolic regulation, Channa punctata species of fish were exposed to cold (4~8°C) for 30 min, 1 hr and 2 hrs since they are much energetic and survive in the critical environment. The inorganic phosphate (Pi) in skeletal muscle was significantly increased after 1 hr in cold compared to control fish kept in ambient temperature, however, although lower, stimulatory response to cold for 2 hrs was also observed. In separate examinations, Na+-K+ ATPase activity in the extract of skeletal muscle was determined and enhanced significantly after 1 hr and 2 hrs. The results appear to indicate that the increased Pi in response to cold might be due to the higher activity of Na+-K+ ATPase. To clarify the regulatory mechanism of the enzyme, groups of fish were exposed to cold with 100 mM Na2HAsO4 for 1 hr and 2 hrs. The enhanced Na+-K+ ATPase activity was reduced significantly when treated with Na2HAsO4 compared to the control and also to the respective cold exposed fish. The experimental findings indicate that arsenic is involved in the regulation of cold induced Na+-K+ ATPase activity. The enhanced Na+-K+ ATPase activity in skeletal muscle of this species in cold and its inhibition by arsenic may play a critical role in environment.

Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa punctata)

Year 2011, Volume: 11 Issue: 3, - , 01.06.2011

Abstract

To characterize whether environmental low temperature is involved in metabolic regulation, Channa punctata species of fish were exposed to cold (4~8°C) for 30 min, 1 hr and 2 hrs since they are much energetic and survive in the critical environment. The inorganic phosphate (Pi) in skeletal muscle was significantly increased after 1 hr in cold compared to control fish kept in ambient temperature, however, although lower, stimulatory response to cold for 2 hrs was also observed. In separate examinations, Na+-K+ ATPase activity in the extract of skeletal muscle was determined and enhanced significantly after 1 hr and 2 hrs. The results appear to indicate that the increased Pi in response to cold might be due to the higher activity of Na+-K+ ATPase. To clarify the regulatory mechanism of the enzyme, groups of fish were exposed to cold with 100 mM Na2HAsO4 for 1 hr and 2 hrs. The enhanced Na+-K+ ATPase activity was reduced significantly when treated with Na2HAsO4 compared to the control and also to the respective cold exposed fish. The experimental findings indicate that arsenic is involved in the regulation of cold induced Na+-K+ ATPase activity. The enhanced Na+-K+ ATPase activity in skeletal muscle of this species in cold and its inhibition by arsenic may play a critical role in environment.

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Details

Primary Language Turkish
Journal Section Articles
Authors

Md. Shahidul Haque This is me

Swapan Kumar Roy This is me

M. Shahjahan This is me

Publication Date June 1, 2011
Published in Issue Year 2011 Volume: 11 Issue: 3

Cite

APA Haque, M. S., Roy, S. K., & Shahjahan, M. (2011). Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa punctata). Turkish Journal of Fisheries and Aquatic Sciences, 11(3).
AMA Haque MS, Roy SK, Shahjahan M. Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa punctata). Turkish Journal of Fisheries and Aquatic Sciences. June 2011;11(3).
Chicago Haque, Md. Shahidul, Swapan Kumar Roy, and M. Shahjahan. “Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa Punctata)”. Turkish Journal of Fisheries and Aquatic Sciences 11, no. 3 (June 2011).
EndNote Haque MS, Roy SK, Shahjahan M (June 1, 2011) Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa punctata). Turkish Journal of Fisheries and Aquatic Sciences 11 3
IEEE M. S. Haque, S. K. Roy, and M. Shahjahan, “Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa punctata)”, Turkish Journal of Fisheries and Aquatic Sciences, vol. 11, no. 3, 2011.
ISNAD Haque, Md. Shahidul et al. “Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa Punctata)”. Turkish Journal of Fisheries and Aquatic Sciences 11/3 (June 2011).
JAMA Haque MS, Roy SK, Shahjahan M. Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa punctata). Turkish Journal of Fisheries and Aquatic Sciences. 2011;11.
MLA Haque, Md. Shahidul et al. “Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa Punctata)”. Turkish Journal of Fisheries and Aquatic Sciences, vol. 11, no. 3, 2011.
Vancouver Haque MS, Roy SK, Shahjahan M. Arsenic Impairs the Effect of Low Temperature on the Regulation of Na+-K+ ATPase Activity in Skeletal Muscle of Fish (Channa punctata). Turkish Journal of Fisheries and Aquatic Sciences. 2011;11(3).