SU ARACILIĞIYLA SODYUM FLORÜR KONSANTRASYONLARINA MARUZ KALMA LEYDİG HÜCRELERİNDE SİTOTOKSİSİTE VE OKSİDATİF HASARA NEDEN OLABİLİR

Year 2017, , 115 - 122, 22.09.2017

Yasemin Aydin

https://doi.org/10.23902/trkjnat.303729

Abstract

Bu çalışma sodyum florürün
(NaF) TM3 Leydig hücrelerinde in vitro’da
sitotoksisite ve oksidatif stres üzerindeki etkilerini araştırmıştır. Sitotoksisite
hücre canlılık ve çoğalma hızı ile ve laktat dehidrogenaz testi ile
belirlenmiştir. Oksidatif hasar, artan lipid peroksidasyon ürünü, reaktif
oksijen türleri (ROS) ve azalan hücresel enzimatik ve enzimatik olmayan
antioksidanlar yoluyla ölçülmüştür. Leydig hücreleri, Türkiye'de içme suyunun
günlük alımına dayanan en düşük ve en yüksek florür konsantrasyonları olan iki
farklı NaF (2ppm ve 16ppm) konsantrasyonuna 24 saat, 48 saat ve 72 saat maruz
bırakılmıştır. Bu çalışma, NaF'ye maruz bırakılan Leydig hücrelerinde,
konsantrasyon ve zamana bağlı olarak, hücre canlılığı ve proliferasyonundaki
azalmanın ve oksidatif stresin arttığını göstermiştir. Bu sonuçlar florür
uygulanmasının Leydig hücrelerinde ve özellikle hücrelerin antioksidan
sisteminde hasara neden olabileceğini önerir. Bu aynı zamanda oksidatif stresin
Leydig hücresi fonksiyonu üzerinde bozulmalarda önemli bir rol oynadığını
kanıtlamaktadır. 

Keywords

Florür, sitotoksisite, lipid peroksidasyonu, ROS, antioksidan sistem, Leydig hücreleri

EXPOSURE TO SODIUM FLUORIDE VIA DRINKING WATER CAUSE CYTOTOXICITY AND OXIDATIVE DAMAGE IN LEYDIG CELLS

Abstract

This study investigated the in vitro effects of sodium fluoride (NaF) on cytotoxicity and
oxidative stress in TM3 Leydig cells. Cytotoxicity was determined with cell
viability and proliferation rate, and lactate dehydrogenase leakage assay.
Oxidative damage was measured by increasing lipid peroxidation product,
reactive oxygen species (ROS) and decreasing cellular enzymatic and
non-enzymatic antioxidants. Leydig cells were exposed to two different
concentrations of NaF (2ppm and 16ppm) for 24h, 48h and 72h which are
considered to be the lowest and highest fluoride concentrations based on daily
intake of drinking water in Turkey. The present study showed that cell
viability and proliferation decreased and oxidative stress increased in
NaF-exposed Leydig cells in a concentration- and time-dependent manner. The
findings suggest that fluoride-treatment can cause damage in Leydig cells,
especially in antioxidant system of the cells. The results also proved that
oxidative stress plays an important role in impairments on Leydig cell function.

Keywords

Fluoride, cytotoxicity, lipid peroxidation, antioxidant system, Leydig cell, reactive oxygen species

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