UPREGULATION OF NEUROGLOBIN PROMOTES TM3 LEYDIG CELL VIABILITY

Year 2018, , 115 - 120, 15.10.2018

Aysegul Dogan

https://doi.org/10.23902/trkjnat.393371

Abstract

Neuroglobin (NGB) is a globin family member protein
expressed in the nerve system and prevents oxidative stress and apoptosis
induced death in neurons. The anti-oxidant role of NGB against oxidative stress
and reactive oxygen species (ROS) brings up its promising protective role in
other tissues. The overexpression of NGB in testosterone producing Leydig cells
might be a solution to hypoxia related male infertility. In the current study, NGB was
overexpressed in Leydig cells by using viral transduction methods and the cell
proliferation, gene expression and anti-oxidant enzyme levels were analyzed.
Upregulation of NGB increased anti-apoptotic Bcl-2, cell proliferation and
anti-oxidant enzyme levels and decreased the expression of apoptotic genes BAX,
p53 and caspase 3. In addition, NGB transduced cells proliferated and expressed
less apoptotic genes after H₂O₂ exposure. In conclusion, NGB might be a
target for androgen deficiency related male infertility and could be used in
clinics in the future.

Keywords

Neuroglobin, Leydig cell, oxidative stress, anti-oxidant enzymes, male fertility

UPREGULATION OF NEUROGLOBIN PROMOTES TM3 LEYDIG CELL VIABILITY

Abstract

Globin ailesi proteinlerinden bir tanesi olan
Neuroglobin (NGB) sinir sisteminde
ifade edilmektedir ve oksidatif stres ve apoptoz kaynaklı nöron ölümünü
engellemektedir. Oksidatif stres ve reaktif oksijen türlerine karşı gözlemlenen
anti-oksidan aktivite, NGB’nin diğer dokulardaki potansiyel koruyucu rolünü
gündeme getirmektedir. Testosteron üreten Leydig hücrelerinde NGB’nin fazla
ifade edilmesi hipoksi ile ilişkili erkek infertilitesi için bir çözüm
olabilecektir. Bu çalışmada, NGB proteinin viral aktarım metodu ile Leydig
hücrelerinde aşırı ifade ettirilmiş ve hücre çoğalması, gen anlatımı ve
anti-oksidan enzim seviyeleri analiz edilmiştir. Artan NGB, anti-apoptotik Bcl-2,
hücre çoğalması ve anti-oksidan enzim seviyelerini arttırmış, BAX, p53 ve
kaspaz 3 gibi apoptoz genlerinin ifadesini azaltmıştır. Ek olarak, H₂O₂
uygulaması sonrasında NGB aktarılan hücreler çoğalmış ve apoptotik
genlerin ifadesi azalmıştır. Sonuç olarak, NGB androjen eksikliğine bağlı erkek
infertilitesi için hedef olabilir ve gelecekte klinikte kullanılabilir.

Keywords

Neuroglobin, Leydig cell, oxidative stress, anti-oxidant enzymes, male fertility

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