Poecilimon zonatus Bolívar (ORTHOPTERA, TETTIGONIIDAE) REVISITED: GENETIC DATA REVEALED TWO NEW SPECIES AND ONE NEW SUBSPECIES

Year 2018, , 85 - 99, 12.04.2018

Sarp Kaya

https://doi.org/10.23902/trkjnat.411785

Cited By: 3

Abstract

Poecilimon Fischer (Orthoptera,
Tettigoniidae) is the most
species-rich genus of the respective order and family in Anatolia. However,
intrageneric diversity still needs to be documented. Poecilimon zonatus Bolívar is a species with a relatively wide
range of distribution in Anatolia compared to other congeners. The present
study aims to examine the diversity of P.
zonatus using an integrated approach. The diversity of the species was documented using
data based on DNA sequences, male calling song and morphology. Automatic
species delimitation tests applied to genetic data revealed a cryptic diversity
in P. zonatus complex and suggested
two new species and one new subspecies. The song data supported the new species,
but morphology remained less productive in this respect. The new taxa defined
in the light of the obtained data are; Poecilimon
isozonatus
sp. n., Poecilimon ciplaki sp. n. as Poecilimon ciplaki ciplaki subsp. n. and
Poecilimon ciplaki denizliensis subsp.
n. These taxa together with P. zonatus
were considered a species complex within P.
zonatus group. The phylogenetic tree produced from nad2 gene sequences supported the following
relationships of P. tauricola + (P.
ciplaki + (P. zonatus +P.
isozonatus))
relationship.

Keywords

Anatolia, Poecilimon isozonatus sp. n., Poecilimon ciplaki sp. n., Poecilimon ciplaki ciplaki subsp. n., Poecilimon ciplaki denizliensis subsp. n., phylogeny

Poecilimon zonatus Bolívar (ORTHOPTERA, TETTIGONIIDAE) REVISITED: GENETIC DATA REVEALED TWO NEW SPECIES AND ONE NEW SUBSPECIES

Abstract

Poecilimon
Fischer (Orthoptera, Tettigoniidae), dahil
olduğu takım ve familyanın Anadolu’da tür sayısı en fazla olan cinstir. Ancak
cins içi çeşitlilik halen ortaya konmayı beklemektedir. Cinsin diğer türleri
ile karşılaştırıldığında Poecilimon
zonatus Bolívar
Anadolu’da nispeten geniş yayılışa sahip bir türdür.
Bu çalışma entegre bir yaklaşımla türün çeşitliliğini saptamayı amaçlamaktadır.
DNA dizileri, erkek çağrı sesi ve morfolojiden üretilen verilerle P. zonatus türünün çeşitliliği
tanımlanmıştır. Genetik verilere uygulanan otomatik tür sınırları testleri P. zonatus’un kriptik bir çeşitlilik
içerdiğini ve iki yeni tür ile bir yeni alttürün varlığına işaret etmiştir.
Erkek çağrı sesi verileri yeni türleri desteklemiştir. Ancak, bu açıdan
morfoloji daha az kullanışlı olmuştur. Elde edilen bu veriler ışığında
tanımlanan yeni taksonlar şunlardır; Poecilimon isozonatus sp. n. Poecilimon ciplaki sp. n. ve Poecilimon ciplaki ciplaki subsp. n. ve Poecilimon
ciplaki denizliensis subsp. n. Bu yeni taksonlar P. zonatus ile birlikte P.
zonatus kompleksi olarak tanımlanmış ve bu kompleksin P. zonatus tür grubu içinde yer aldığı sonucuna varılmıştır. nad2 gen dizilerinden üretilen filogenetik ağaç P. tauricola + (P.
ciplaki + (P. zonatus +P. isozonatus)) akrabalık ilişkisini desteklemiştir.

Keywords

Anatolia, Poecilimon isozonatus sp. n., Poecilimon ciplaki sp. n., Poecilimon ciplaki ciplaki subsp. n., Poecilimon ciplaki denizliensis subsp. n., phylogeny.

References

• 1. Bey-Bienko, G.Y. 1954. Orthoptera. Leaf bush-crickets (Phaneropterinae). Fauna of the USSR, Vol. II, Sect. 2. Zoological Institute of the Academy of Sciences of the USSR, New Series, 59, 385 pp. (in Russian)
• 2. Cigliano, M.M., Braun, H., Eades, D.C. & Otte, D. 2018. Orthoptera Species File. Version 5.0/5.0. http://Orthoptera.SpeciesFile.org (Date accessed: March 2018).
• 3. Chobanov, D.P., Kaya, S., Grzywacz, B., Warchałowska-Śliwa, E. & Çıplak, B. 2017. The Anatolio-Balkan phylogeographic fault: a snapshot from the genus Isophya (Orthoptera, Tettigoniidae). Zoologica Scripta, 46(2): 165-179 (doi:10.1111/zsc.12194). 4. Çıplak, B. 2003. Distribution of Tettigoniinae (Orthoptera, Tettigoniidae) bush-crickets in Turkey: the importance of the Anatolian Taurus Mountains in biodiversity and implications for conservation. Biodiversity and Conservation, 12: 47-64.
• 5. Çıplak, B. 2004. Biogeography of Anatolia: the marker group Orthoptera. Memorie della Società Entomologica Italiana, 82: 357-372.
• 6. Çıplak, B. 2008. The analogy between glacial cycles and global warming for the glacial relicts in a refugium: a biogeographic perspective for conservation of Anatolian Orthoptera, pp. 135-163, in: Fattorini, S. (ed) Insect Ecology and Conservation. Research Signpost, Kerela, India.
• 7. Çıplak, B., Demirsoy, A. & Bozcuk, A.N. 1993. Distribution of Orthoptera in relation to the Anatolian Diagonal in Turkey. Articuluta, 8: 1-20.
• 8. Çıplak, B., Demirsoy, A. & Bozcuk, A.N. 1996. Malatya Ensifera (Orthoptera, Insecta) faunası. Turkish Journal of Zoology, 20: 247-254.
• 9. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9: 772.
• 10. Harz, K. 1969. Die Orthopteren Europas 1. Series Entomologica 5, The Hague (Dr. W. Junk N.V.), 749 pp.
• 11. Heller, K.-G. 1988. Bioakustik der europäischen Laubheuschrecken. Verlag Josef Margraf, Weikersheim, 358 pp.
• 12. Kaya, S., Çıplak, B., Chobanov, D. & Heller, K-G. 2012. Poecilimon bosphoricus group (Orthoptera, Phaneropterinae): iteration of morpho-taxonomy by song characteristics. Zootaxa, 3225, 1-71. 13. Kaya, S. & Çıplak, B. 2017. Phylogeography and taxonomy of the Psorodonotus caucasicus (Orthoptera, Tettigoniidae) group:independent double invasion of the Balkans from the Caucasus. Systematic Entomology, 42: 118-133.
• 14. Kaya, S., Boztepe, Z. & Çıplak, B. 2015. Phylogeography of the Poecilimon luschani species group (Orthoptera, Tettigoniidae): a radiation strictly correlated with climatic transitions in the Pleistocene. Zoological Journal of the Linnean Society, 173(1): 1-21.
• 15. La Greca M. 1999. Il contributo degli Ortotteri (Insecta) alla conoscenza della biogeografia dell’Anatolia: la componente gondwaniana. Biogeographia, 20: 179-200.
• 16. Librado, P. & Rozas, J. 2009. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25: 1451-1452.
• 17. Miram, E.F. 1938. New species of the genera Poecilimon Fisch. Fr. and Isophya Br. W (Subf. Phaneropterinae Fam. Tettigonoidea - long-horned grasshoppers) of the fauna of the USSR. Zoologicheskii zhurnal, 17: 348-372.
• 18. R Development Core Team 2018. R: A language and environment for statistical computing, Version 3.3.4. - Available at http://www.R-project.org
• 19. Ragge, D.R. & Reynolds, W.J. 1998. The Songs of the Grasshoppers and Crickets of Western Europe. Harley Books, London, 612 pp. 20. Ramme, W. 1933. Revision der Phaneropterinen-Gattung Poecilimon Fisch. (Orth. Tettigon). Mitteilungen aus dem Zoologischen Museum in Berlin, 19: 497-575.
• 21. Ramme, W. 1951. Zur Systematik, Faunistik und Biologie der Orthopteren von Südost-Europa und Vorderasien. Mitteilungen aus dem Zoologischen Museum in Berlin, 27: 1-421.
• 22. Silvestro, D. & Michalak, I. 2012. raxmlGUI: a graphical front-end for RAxML. Organisms Diversity & Evolution 12: 335-337.
• 23. Simon, C., Buckley, T.R., Frati, F., Stewart, J.B. & Beckenbach, A.T. 2006. Incorporating Molecular Evolution into Phylogenetic Analysis, and a New Compilation of Conserved Polymerase Chain Reaction Primers for Animal Mitochondrial DNA. Annual Review of Ecology, Evolution and Systematics, 37: 545-579.
• 24. Sevgili, H., Demirsoy, A. & Durmuş, Y. 2012. Orthoptera fauna of Kemaliye (Erzincan). Hacettepe Journal of Biology and Chemistry, 40(4): 317-335.
• 25. Sueur J., Aubin T. & Simonis C. 2008. Seewave: a free modular tool for sound analysis and synthesis. Bioacoustics, 18: 213-226.
• 26. Ullrich, B., Reinhold, K., Niehuis, O. & Misof, B. 2010. Secondary structure and phylogenetic analysis of the internal transcribed spacers 1 and 2 of bush crickets (Orthoptera: Tettigoniidae: Barbitistini). Journal of Zoological Systematics and Evolutionary Research, 48: 219-228.
• 27. Ünal, M. 2010. Phaneropterinae (Orthoptera: Tettigoniidae) from Turkey and the Middle East II. Transactions of the American Entomological Society, 136: 125-183.