THE EFFECTS OF VITAMIN C ON GLYCIDAMIDE-INDUCED CELLULAR DAMAGE AND APOPTOSIS IN MOUSE LEYDIG CELLS

Year 2019, , 9 - 17, 15.04.2019

Banu Orta Yılmaz

https://doi.org/10.23902/trkjnat.454020

Abstract

The aim of this study was to elucidate the role
of vitamin C on glycidamide-induced cytotoxicity, oxidative damage and cell
death in Leydig (TM3) cells. Leydig cells were exposed to glycidamide (1, 10,
100 and 1000 μM) and/or vitamin C (50 μM) for 24 h. After completion of the
exposure time, cell viability, amount of lactate dehydrogenase enzyme,
apoptosis-necrosis rates, levels of oxidative stress parameters such as
hydroxyl radical, hydrogen peroxide and lipid peroxidation were determined in
Leydig cells. The results showed that glycidamide administration decreased
Leydig cell viability and increased cytotoxicity significantly at high
concentration (1000 μM). In addition, glycidamide generated oxidative damage by
significantly increasing the production of reactive oxygen species and lipid
peroxidation. Exposure to glycidamide increased the formation of early
apoptosis, apoptosis and necrosis in Leydig cells. Consequently, glycidamide
has been shown to cause apoptosis due to lipid peroxidation and formation of
reactive oxygen species in Leydig cells, and vitamin C has a therapeutic role
against toxicity caused by glycidamide.

Keywords

Glycidamide, cytotoxicity, oxidative stress, Vitamin C, Leydig cell, apoptosis

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