Öz

Bu çalışmada Afyonkarahisar'da yetiştirilen Salvia officinalis L. (Lamiaceae) uçucu yağ oranı ve kalitesi üzerindeki fenolojik evre ve diurnal varyabilite etkileri araştırılmıştır. Ontogenetik varyabiliteye dayalı hasat zamanları çiçeklenme öncesi, çiçeklenme dönemi ve çiçeklenme sonrası olarak belirlenmiştir. Diurnal varyabilite olarak hasat saatleri 06:00, 14:00 ve 20:00 ayarlanmıştır. Sonuçlara göre en yüksek uçucu yağ çiçeklenme döneminde ve saat 20:00'de (%2,05) elde edilmiştir. En iyi hasat zamanı çiçeklenme dönemi ve günün son saatleri olarak saptanmıştır. Diğer fenolojik evrelerde uçucu yağ sentezi neredeyse aynı oranda bulunmuştur. Ayrıca, çalışılan tüm evrelerde uçucu yağ içeriği günün son saatlerine doğru artmıştır. Salvia officinalis uçucu yağının tanımlanan bileşikleri arasında oksijenli monoterpenler en büyük kimyasal gruba sahip olmuştur (%52,8-68,6). α-Thujone (%13,0-35,8) deneme örneklerinin çoğunda ana bileşik olarak belirlenmiştir. Bu bileşiğin en yüksek ve en düşük değerleri sırasıyla çiçeklenme sonrası ve çiçeklenme aşamalarında gözlenmiştir. Camphor (%7,0-20,2), 1,8-sineol (%6,9-14,1), borneol (%2,8-15,8) ve veridiflorol (%4,5-12,3) diğer önemli bileşikler olarak tanımlanmıştır. Salvia officinalis uçucu yağının miktarı ve kalitesi üzerinde gün uzunluğu, güneşlenme, sıcaklık gibi iklim faktörleri ve bitki büyüme evresi etkileri belirgin bir şekilde ortaya çıkmıştır. Bu çalışmanın sonuçları doğrultusunda, S. officinalis’in en uygun hasat zamanı Afyonkarahisar koşullarında çiçeklenme dönemi ve saat 20:00 olarak belirlemiştir. Bitkinin uçucu yağ bileşenleri, farklı hasat zamanlarında (ontogenetik ve diurnal) geniş değişim göstermiştir.

PHENOLOGICAL CYCLE AND DIURNAL VARIATION EFFECTS ON THE VOLATILE OIL CHARACTERISTICS OF SAGE (Salvia officinalis L.)

Abstract

This study was carried out to evaluate the effects of phenological and diurnal variation on volatile oil content and quality of Salvia officinalis L. (Lamiaceae) cultivated in Afyonkarahisar/Turkey. The harvesting times based on ontogeny were the pre-flowering, flowering and post-flowering stages. The harvesting took place three times a day, at 6:00 a.m., 2:00 and 8:00 p.m. The results showed that the highest volatile oil was obtained at 8:00 p.m. of the flowering stage (2.05%). In general, the best harvesting time was the flowering stage and the last hours of the day. The synthesis of volatile oil was almost the same at other phenological stages. The amount of volatile oil increased at the sunset time of the day in all studied stages. Among the identified of S. officinalis volatile oil compounds, oxygenated monoterpenes were the largest chemical group (52.8-68.6%). α-Thujone (13.0-35.8%) was the major compound of the most samples. The highest and lowest values of this compound were observed in the post-flowering and flowering stages, respectively. The other main compounds were camphor (7.0-20.2%), 1,8-cineole (6.9-14.1%), borneol (2.8-15.8%) and veridiflorol (4.5-12.3%). The effects of climatic factors such as day length, insolation, temperature and plant growth stage affected the quantity and quality of volatile oil content of S. officinalis. The results showed that the best harvesting time for S. officinalis for volatile oil content is 8:00 p.m. at the flowering stage in Afyonkarahisar climatic conditions. The volatile oil compositions of the plant varied widely at different harvest times (ontogeny and diurnal).

Keywords

• Diurnal
• Essential oil
• GC-MS
• Ontogeny
• Salvia officinalis

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