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Purification of Some Grape Cultivar (Vitis vinifera L.) and Rootstock Clones Eliminated from Viruses with Thermotherapy and Meristem Culture

Year 2015, Volume: 16 Issue: 2, 57 - 61, 15.02.2016

Abstract

Turkey, one of the few important countries in the world concerning the total area of the vineyards and grape
production is not in a desired level considering the amount of grape yield per unit area. The use of materials of unknown quality
and whose disease and pest related conditions are not taken into account in sapling production, which is the basic material of
grape production, is the main cause of this fact. Plant pathogen viruses, for which no cultural or chemical fight against are
unknown, are more important than other disease and pest organisms during sapling production processes. Elimination of the
viruses from the base materials used in sapling production in viticulture is only possible with thermotherapy and meristem
culture. In this study, the clones of fourteen grape varieties (Vitis vinifera L.) and six rootstocks that have economic importance
for our country were used to purify from Agrobacterium vitis and viruses specified in the certification system of our country
and European Union countries using thermotherapy and meristem culture methods. 93 plants were obtained at the end of
purification processes of the studied clones grape cultivars and rootstocks.

References

  • Anonim, 2005. Faostat Statistical Database.
  • Barlass, M., Skene, K.G.M. & Clingelefter, P.R. 1982. Studies on the Fragmented Shoot Apex of Grapevine III. A Scanning Elektron Microscope Study Of Adventitious Bud Formation in Vitro. Horticultural Science Abstracts, 52(3):138.
  • Benlioğlu, K. & Özakman, M., 1998. Bağ Üretim Materyalinde Kök Uru Etmeni Agrobacterium tumefaciens'in saptanması. Tarım ve Ormancılık Dergisi, 22: 167-174.
  • Murashige. T. & Skoog, F.A. 1962. Revised medium for rapid growth and bio-qqassays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
  • Söylemezoğlu, G., Kunter, B., Akkurt, M., Sağlam, M., Ünal, A., Buzrul, S. & Tahmaz, H. 2015. Bağcılığın Geliştirilmesi Yöntemleri ve Üretim Hedefleri. Türkiye Ziraat Mühendisliği VIII. Teknik Kongresi Bildiriler Kitabı, 1 Ocak, Ankara.
  • Stellmach, G. 1980. Moderate heat propogation of grapevines for eliminating graft transmissible disorders. 325-328. Proceedings of the 7th Meeting of the International Council for the Study of Viruses and Virus-like Diseases of the Grapevine. Vineland Research Station, Ontario Canada. 8-12 Eylül 1980.

BAZI ASMA (Vitis vinifera L.) ÇEŞİT ve ANAÇ KLONLARININ TERMOTERAPİ ve MERİSTEM KÜLTÜRÜ İLE VİRÜSLERDEN ARINDIRILMASI

Year 2015, Volume: 16 Issue: 2, 57 - 61, 15.02.2016

Abstract

Bağ alanları ve toplam üzüm üretimi bakımından dünyanın sayılı ülkeleri arasında yer alan Türkiye, birim alandan elde edilen üzüm miktarı göz önüne alındığında istenilen düzeyde bulunmamaktadır. Bu durumun en önemli nedenlerinin başında, üzüm üretiminin temel materyali olan fidanın üretimi sırasında ülkemizde genellikle kalite özellikleri belli olmayan, hastalık ve zararlılarla ilgili durumu dikkate alınmayan materyallerin kullanılması gelmektedir. Fidan üretim sürecinde, bilinen herhangi bir kültürel veya kimyasal mücadelesi olmayan bitki patojeni virüsler, diğer hastalık ve zararlılar arasında ön planda bulunmaktadır. Bağcılıkta fidan üretiminde kullanılacak olan baz materyallerin virüslerden arındırılması işlemi termoterapi ve beraberinde meristem kültürü uygulamaları ile mümkün olmaktadır. Bu çalışmada ülkemiz için ekonomik önemi bulunan 14 asma çeşit (Vitis vinifera L.) ve 6 anacına ait klonlar kullanılarak Avrupa Birliği ülkeleri ve ülkemiz sertifikasyon sisteminde belirlenmiş olan virüslerden ve asma patojeni Agrobacterium vitis'ten, termoterapi ve meristem kültürü yöntemleri ile arındırma yapılmıştır. Arındırma işlemleri sonucunda çalışılan asma çeşit ve anaç klonlarından 93 bitki elde edilmiştir.

References

  • Anonim, 2005. Faostat Statistical Database.
  • Barlass, M., Skene, K.G.M. & Clingelefter, P.R. 1982. Studies on the Fragmented Shoot Apex of Grapevine III. A Scanning Elektron Microscope Study Of Adventitious Bud Formation in Vitro. Horticultural Science Abstracts, 52(3):138.
  • Benlioğlu, K. & Özakman, M., 1998. Bağ Üretim Materyalinde Kök Uru Etmeni Agrobacterium tumefaciens'in saptanması. Tarım ve Ormancılık Dergisi, 22: 167-174.
  • Murashige. T. & Skoog, F.A. 1962. Revised medium for rapid growth and bio-qqassays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
  • Söylemezoğlu, G., Kunter, B., Akkurt, M., Sağlam, M., Ünal, A., Buzrul, S. & Tahmaz, H. 2015. Bağcılığın Geliştirilmesi Yöntemleri ve Üretim Hedefleri. Türkiye Ziraat Mühendisliği VIII. Teknik Kongresi Bildiriler Kitabı, 1 Ocak, Ankara.
  • Stellmach, G. 1980. Moderate heat propogation of grapevines for eliminating graft transmissible disorders. 325-328. Proceedings of the 7th Meeting of the International Council for the Study of Viruses and Virus-like Diseases of the Grapevine. Vineland Research Station, Ontario Canada. 8-12 Eylül 1980.
There are 6 citations in total.

Details

Subjects Structural Biology
Journal Section Research Article/Araştırma Makalesi
Authors

Onur Ergönül This is me

Lerzan Öztürk This is me

Publication Date February 15, 2016
Submission Date October 8, 2014
Published in Issue Year 2015 Volume: 16 Issue: 2

Cite

APA Ergönül, O., & Öztürk, L. (2016). Purification of Some Grape Cultivar (Vitis vinifera L.) and Rootstock Clones Eliminated from Viruses with Thermotherapy and Meristem Culture. Trakya University Journal of Natural Sciences, 16(2), 57-61.
AMA Ergönül O, Öztürk L. Purification of Some Grape Cultivar (Vitis vinifera L.) and Rootstock Clones Eliminated from Viruses with Thermotherapy and Meristem Culture. Trakya Univ J Nat Sci. March 2016;16(2):57-61.
Chicago Ergönül, Onur, and Lerzan Öztürk. “Purification of Some Grape Cultivar (Vitis Vinifera L.) and Rootstock Clones Eliminated from Viruses With Thermotherapy and Meristem Culture”. Trakya University Journal of Natural Sciences 16, no. 2 (March 2016): 57-61.
EndNote Ergönül O, Öztürk L (March 1, 2016) Purification of Some Grape Cultivar (Vitis vinifera L.) and Rootstock Clones Eliminated from Viruses with Thermotherapy and Meristem Culture. Trakya University Journal of Natural Sciences 16 2 57–61.
IEEE O. Ergönül and L. Öztürk, “Purification of Some Grape Cultivar (Vitis vinifera L.) and Rootstock Clones Eliminated from Viruses with Thermotherapy and Meristem Culture”, Trakya Univ J Nat Sci, vol. 16, no. 2, pp. 57–61, 2016.
ISNAD Ergönül, Onur - Öztürk, Lerzan. “Purification of Some Grape Cultivar (Vitis Vinifera L.) and Rootstock Clones Eliminated from Viruses With Thermotherapy and Meristem Culture”. Trakya University Journal of Natural Sciences 16/2 (March 2016), 57-61.
JAMA Ergönül O, Öztürk L. Purification of Some Grape Cultivar (Vitis vinifera L.) and Rootstock Clones Eliminated from Viruses with Thermotherapy and Meristem Culture. Trakya Univ J Nat Sci. 2016;16:57–61.
MLA Ergönül, Onur and Lerzan Öztürk. “Purification of Some Grape Cultivar (Vitis Vinifera L.) and Rootstock Clones Eliminated from Viruses With Thermotherapy and Meristem Culture”. Trakya University Journal of Natural Sciences, vol. 16, no. 2, 2016, pp. 57-61.
Vancouver Ergönül O, Öztürk L. Purification of Some Grape Cultivar (Vitis vinifera L.) and Rootstock Clones Eliminated from Viruses with Thermotherapy and Meristem Culture. Trakya Univ J Nat Sci. 2016;16(2):57-61.

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