Abstract
Interest in lipases has markedly increased to their potential industrial applications. The
most of lipases produced commercially are obtained from animal and microbial sources.
Nowadays, also obtained from plant seeds such as sunflower, soybean, peanut, castor bean and
hazelnut. Hazelnut is one of the most important foods in majority of the world and Turkey is
largest hazelnut producer. In this study, It was aimed that Lipase from hazelnut seed identified as
yomra species isolated, purified and characterized. Lipase from hazelnut seed was purified 1255
fold to homogeneous state by ammonium sulfate precipitation, dialysis and Sephadex G-100 gel
filtration chromatography after by defatting from hazelnut proteins. The purified enzyme
showed single band when it was subjected to SDS-PAGE. The molecular weight of the
determined by SDS-PAGE was 20 kDa. Purified lipase from hazelnut seed exhibited the
maximum activity at 9.0 and 50˚C and stable under alkaline conditions (pH 7.0-10.0) and at
temperatures between 20-55˚C. Lipase from hazelnut seed more specified versus triolein and
tributyrin and olive oil among the nature oils as substrate. The enzyme activity was measured by
using 0.1 ml of enzyme solution for 5 min. To determine the storage stability of lipase from
hazelnut seed, the activity assays carried out for a period of one year. it was observed that about
83% of its activity was retained of 9 months at -20˚C. Purified lipase from hazelnut seed versus
triolein as substrate calculated Km and Vmax values, 4.545mM and 80 U/dk.mg.Enzyme,
respectively.
Abstract
Interest in lipases has markedly increased to their potential industrial applications. The
most of lipases produced commercially are obtained from animal and microbial sources.
Nowadays, also obtained from plant seeds such as sunflower, soybean, peanut, castor bean and
hazelnut. Hazelnut is one of the most important foods in majority of the world and Turkey is
largest hazelnut producer. In this study, It was aimed that Lipase from hazelnut seed identified as
yomra species isolated, purified and characterized. Lipase from hazelnut seed was purified 1255
fold to homogeneous state by ammonium sulfate precipitation, dialysis and Sephadex G-100 gel
filtration chromatography after by defatting from hazelnut proteins. The purified enzyme
showed single band when it was subjected to SDS-PAGE. The molecular weight of the
determined by SDS-PAGE was 20 kDa. Purified lipase from hazelnut seed exhibited the
maximum activity at 9.0 and 50˚C and stable under alkaline conditions (pH 7.0-10.0) and at
temperatures between 20-55˚C. Lipase from hazelnut seed more specified versus triolein and
tributyrin and olive oil among the nature oils as substrate. The enzyme activity was measured by
using 0.1 ml of enzyme solution for 5 min. To determine the storage stability of lipase from
hazelnut seed, the activity assays carried out for a period of one year. it was observed that about
83% of its activity was retained of 9 months at -20˚C. Purified lipase from hazelnut seed versus
triolein as substrate calculated Km and Vmax values, 4.545mM and 80 U/dk.mg.Enzyme,
respectively.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Article/Araştırma Makalesi |
| Authors | |
| Publication Date | June 1, 2012 |
| Submission Date | August 21, 2014 |
| Published in Issue | Year 2012 Volume: 13 Issue: 1 |
Cite
You can reach the journal's archive between the years of 2000-2011 via https://dergipark.org.tr/en/pub/trakyafbd/archive (Trakya University Journal of Natural Sciences (=Trakya University Journal of Science)
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