ADMINISTRATION OF BONE MARROW DERIVED MESENCHYMAL STEM CELLS MODULATE TLR EXPRESSION DURING LIVER REGENERATION

Year 2019, Special Issue: Stem Cell, 1 - 10, 01.02.2019

Hande Kocak Zeynep Tokcaer-keskin Burcu İnsal İhsan Gursel , Kamil Can Akçalı

https://doi.org/10.23902/trkjnat.505243

Abstract

Liver cell transplantation is a powerful alternative to
orthotopic cell transplantation in the treatment of liver failures. Recently,
considerable effort is being channeled to understand the nature and kinetics of
directing stem cells to effectively accumulate at the regenerating liver site.
Mesenchymal stem cells are one of the promising cell sources modulating liver
regeneration process. Present  was
designed to study how mesenchymal stem cells might modulate liver immune
behaviors by changing Toll-like receptor (TLR) expression and increase
regenerative potential during liver regeneration in rats.

Normal and partially hepatectomized rats were treated with
mesenchymal stem cells isolated and expanded from rat bone marrows.
Accumulation of mesenchymal stem cells was confirmed by Real Time-Polymerase
Chain Reaction  (RT-PCR),
Fluorescence-Activated Cell Sorting 
(FACS), and Immunofluorescence Staining (IFS). Student's t-test analysis
was used to evaluate the significance of differences between sham and partially
hepatectomized rat groups.

Our results showed that mesenchymal stem cells expressed
several TLRs, and their accumulation during regeneration was depended on the
timing of injury. Mesenchymal stem cells isolated from bone marrow of normal
rats were observed at the injured liver 3 days after the injection. There were
no labeled mesenchymal stem cells in the liver sections of the uninjured
animals. Mesenchymal stem cell administration significantly altered the
expression of TLR2, 3 and 9 while retaining their migration potential to
regenerating liver.

Our findings implicated that
mesenchymal stem cell administration during liver regeneration modulate the
immune response through changing the expression of the TLRs in the remaining
liver parts into which the cells are recruited or infused. This alteration may
contribute to the regeneration process following partial hepatectomy.

Keywords

Mesenchymal stem cell, TLR, homing, liver regeneration, rat

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