MOLECULAR DYNAMICS STUDIES OF THE NOROVIRUS-HOST CELL INTERACTION MEDIATED BY H-TYPE 1 ANTIGEN

Year 2019, Volume: 20 Issue: 1, 19 - 26, 15.04.2019

Abdulkadir Kocak , Müslüm Yıldız

https://doi.org/10.23902/trkjnat.508120

Cited By: 3

Abstract

Noroviruses are the main cause for acute gastroenteritis disease. They
infect the host cell via interaction with HGBA receptors on the cell surface. Virus makes complex
with cell surface receptors through its capsid protein VP1 to enter the cell.
Although the protein has been successfully crystallized in the presence of some
common glycans, the dynamic change in the protein structure when interacting
with sugar moieties has yet to be fully elucidated. This is critically
important since it leads to understanding the protein’s recognition mechanism of
HBGAs and develop therapeutic strategies against the gastroenteritis disease.
Here, we computationally assessed the dynamic features of wild type VP1
envelope protein to get insights into the interactions that can be important
for virus infectivity. We have found that the binding of sugar moiety does not
cause noticeable dynamic changes in the binding region. However, interestingly,
a drastic change occurs in a distant loop lying at the residue numbers of
395-400, which might be indication of an allosteric effect.

Keywords

Norovirus, therapeutic antibody, HBGA blockage, molecular dynamics

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