FUNCTIONAL CHARACTERIZATION OF SPERMINE FAMILY TRANSPORTER caf5+ IN Schizosaccharomyces pombe (Lindner)

Year 2019, Volume: 20 Issue: 2, 89 - 96, 15.10.2019

Aslıhan Örs Gevrekci

https://doi.org/10.23902/trkjnat.527846

Cited By: 1

Abstract

Polyamines are well conserved
polycationic molecules that are known to interact with nucleic acids and
contribute to multiple functions
including cell cycle and stress response. The transport of polyamines in and
out of the cell is driven by polyamine transporters that play a significant
role in polyamine homeostasis. Schizosaccharomyces pombe (Lindner) caf5⁺
gene codes for a spermine family transporter that is yet to be characterized
functionally. This study aims to understand the contribution of caf5⁺
on different processes previously associated with polyamines, by reverse
genetics. Deletion mutants of caf5⁺, which are viable in
normal conditions, were scanned for multiple cellular processes. The results
showed that caf5⁺ deletion caused shorter cell length and
slightly faster growth rate at the optimum conditions. caf5Δ cells also
showed sensitivity to high doses of UV irradiation, while no sensitivity was
observed against osmotic stress or another DNA damaging agent hydroxyurea. The
mutants could successfully go through different phases of mitosis and meiosis
as observed by DNA and septum staining. In summary, caf5⁺ gene is
involved in normal growth and cell cycle progression, as well as stress
response upon UV irradiation.

Keywords

cell size, cell cycle, stress response, Schizosaccharomyces pombe, polyamine

Supporting Institution

The Scientific and Technological Research Council of Turkey (TÜBİTAK)

Project Number

TÜBİTAK 111T509

Thanks

This work was financially supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK project # 111T509)

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