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FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY

Year 2020, Volume: 21 Issue: 1, 63 - 67, 15.04.2020
https://doi.org/10.23902/trkjnat.688646

Abstract

More than 50 viruses causing infection in members of the family Cucurbitaceae have been identified in the world so far. Because of the development of virus detection methods, new viruses are added to the known infectious cases list every day. One of the viruses recently identified is the Cucumis melo endornavirus (CmEV) which has been reported from different countries all over the world. However, no study for determination of CmEV has been done so far in Turkey. For the purpose of this study, 59 cucurbit plants showing virus and virus-like symptoms were collected from Manisa and İzmir provinces in Turkey. The samples were tested, for presence of CmEV, by reverse-transcriptase polymerase chain reaction and CmEV infections were detected in 47 samples, of which 44 were melon (Cucumis melo Linn.), and the remaining were gherkins (C. anguria Linn.). Among the infected samples, five samples (three were melon, and two were gherkin) were chosen for sequence analysis. After evaluating the sequence analysis results, it was shown that the Turkish isolates presented 93%-99% and 93%-98% identities at the nucleotide level and 94%-99% and 91%-98% identities at the amino acid level among each other and worldwide isolates, respectively. To the best of our knowledge, this is the first report of CmEV in gherkin as a new host both in Turkey and worldwide.

Supporting Institution

Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit

Project Number

FBA-2019-2873

References

  • 1. Baek, D., Lim, S. & Moon J.S. 2016. First report of Cucumis melo endornavirus on Cucumis melo var. makuwa in Korea. https://www.ncbi.nlm.nih.gov/nuccore/KX641269 (Date accessed: 20 December 2019)
  • 2. Çat, A., Yardımcı, N. & Kılıç, H.Ç. 2016. Identification of viral agents in the greenhouses in cucumber and pumpkin in Antalya province and counties. Süleyman Demirel University Journal of Natural and Applied Sciences, 20(1): 129-132.
  • 3. da Costa, A.C., Leal, E., Gill, D., de Pádua Milagres, F.A., Komninakis, S.V., Brustulin, R., Teles, M.A.R, Lobato, M.C.A.B.S., das Chagas, R.T., Abrão, M.F.N.S., Soares, C.V.D.A., Delwart, X.D.E., Sabino E.C. & Luchs, A. 2019. Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil. Virus Genes, 55(3): 332-338.
  • 4. Escalante, C., Sela, N., Dombrovsky, A. & Valverde, R. 2016. Interactions between bell pepper endornavirus, bell pepper, and acute plant viruses. In Proceedings of XVIth EUCARPIA Capsicum and Eggplant Working Group Meeting in Memoriam Dr. Alain Palloix, Kecskemét, Hungary, 231-237.
  • 5. Fukuhara, T., Moriyama, H., Pak, J.K., Hyakutake, T. & Nitta, T. 1993. Enigmatic double-stranded RNA in Japonica rice. Plant Molecular Biology, 21(6): 1121-1130.
  • 6. Fukuhara, T., Koga, R., Aoki, N., Yuki, C., Yamamoto, N., Oyama, N., Udagawa, T., Horiuchi, H., Miyazaki, S., Higashi, Y., Takeshita, M., Ikeda, K., Arakawa, M., Matsumoto, N. & Moriyama, H. 2006. The wide distribution of endornaviruses, large double-stranded RNA replicons with plasmid-like properties. Archives of Virology, 151(5): 995-1002.
  • 7. Fukuhara, T. 2019. Endornaviruses: persistent dsRNA viruses with symbiotic properties in diverse eukaryotes. Virus Genes, 55(2): 165-173.
  • 8. Herschlag, R., Escalante, C., de Souto, E.R., Khankhum, S., Okada, R. & Valverde, R.A. 2019. Occurrence of putative endornaviruses in non-cultivated plant species in South Louisiana. Archives of Virology, 164(7): 1863-1868.
  • 9. Jeffrey, C. 1980. A review of the Cucurbitaceae. Botanical Journal of the Linnean Society, 81(3): 233247.
  • 10. Kamberoglu, M.A., Caliskan, A.F. & Desbiez, C. 2016. Current status of some cucurbit viruses in Çukurova region (Adana and Mersin provinces) of Turkey and molecular characterization of zucchini yellow mosaic virus isolates. Romanian Biotechnological Letters, 21(4): 11709-11719.
  • 11. Kaya, A. & Erkan, S. 2011. Detection and incidence of viruses in cucurbits grown in Izmir, Aydın, Manisa and Balıkesir provinces. Plant Protection Bulletin, 51(4): 387-405.
  • 12. Keçe, M.A. & Kamberoğlu, M.A. 2016. Biological, serological and molecular detection of watermelon mosaic virus (WMV-2) in watermelon growing fields in Eastern Mediterranean region. Journal of Tekirdag Agricultural Faculty, 13(03): 156-164.
  • 13. Köklü, G. & Yilmaz, Ö. 2006. Occurrence of cucurbit viruses on field-grown melon and watermelon in the Thrace region of Turkey. Phytoprotection, 87(3): 123-130.
  • 14. Li, R., Mock, R., Huang, Q., Abad, J., Hartung, J. & Kinard, G. 2008. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. Journal of Virological Methods, 154(1-2): 48-55.
  • 15. Muhire, B.M., Varsani, A. & Martin, D.P. 2014. SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One, 9: 0108277.
  • 16. Okada, R., Kiyota, E., Sabanadzovic, S., Moriyama, H., Fukuhara, T., Saha, P., Roossinck, M.J., Severin, A. & Valverde, R.A. 2011. Bell pepper endornavirus: molecular and biological properties and occurrence in the genus Capsicum. Journal of General Virology, 92(11): 2664-2673.
  • 17. Ozaslan, M., Aytekin, T., Bas, B., Kilic, H., Afacan, I.D. & Dag, D.S. 2006. Virus diseases of cucurbits in Gaziantep, Turkey. The Plant Pathology Journal, 5(1): 24-27.
  • 18. Quito-Avila, D.F., Peralta, E.L., Martin, R.R., Ibarra, M.A., Alvarez, R.A., Mendoza, A., Insuasti, M. & Ochoa, J. 2014. Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region. European Journal of Plant Pathology, 140(2): 193-197.
  • 19. Sabanadzovic, S., Wintermantel, W.M., Valverde, R.A., McCreight, J.D. & Aboughanem-Sabanadzovic, N. 2016. Cucumis melo endornavirus: genome organization, host range and co-divergence with the host. Virus Research, 214: 49-58.
  • 20. Topkaya, S., Desbiez, C. & Ertunc, F. 2019. Presence of cucurbit viruses in Ankara and Antalya province and molecular characterization of coat protein gene of zucchini yellow mosaic virus Turkish isolates. Fresenius Environmental Bulletin, 28(4): 2442-2449.
  • 21. Valverde, R.A., Khalifa, M.E., Okada, R., Fukuhara, T., & Sabanadzovic, S. 2019. ICTV virus taxonomy profile: Endornaviridae. Journal of General Virology, 100(8): 1204-1205.
  • 22. Wakarchuk, D.A. & Hamilton, R.I. 1985. Cellular double-stranded RNA in Phaseolus vulgaris. Plant Molecular Biology, 5(1): 55-63.
  • 23. Zabalgogeazcoa, I.A. & Gildow, F.E. 1992. Double-stranded ribonucleic acid in ‘Barsoy’ barley. Plant Science, 83(2): 187-194.
  • 24. Zitter, T.A., Hopkins, D.L. & Thomas, C.E. 1996. Compendium of Cucurbit Diseases. The American Phytopathological Society Aps Press, St. Paulk, Minesota, 87p.
Year 2020, Volume: 21 Issue: 1, 63 - 67, 15.04.2020
https://doi.org/10.23902/trkjnat.688646

Abstract

Dünyada gerçekleştirilen çalışmalar sonucunda kabakgil familyasındaki bitkileri enfekte eden 50'den fazla virüs varlığı tanımlanmıştır. Virüs tespit metotlarındaki gelişmelere bağlı olarak bu sayıya her geçen gün yenileri eklenmektedir. Son yıllarda tanılanan bu virüs hastalıklarından bir tanesi de Cucumis melo endornavirus (CmEV)'dır. CmEV'nin enfeksiyonu dünyanın farklı ülkelerinden bildirilmiştir. Ancak, ülkemizde şimdiye kadar CmEV varlığının belirlenmesine yönelik bir çalışma gerçekleştirilmemiştir. Bu amaçla, Manisa ve İzmir illerinden virüs ve virüs-benzeri belirti gösteren 59 kabakgil bitkisinden örnekler alınmıştır. Toplanan örnekler ters transkriptaz-polimeraz zincir reaksiyonu ile test edilmiştir. Gerçekleştirilen bu testler sonucunda 47 örnekte CmEV enfeksiyonu tespit edilmiştir. Enfekteli 47 örneğin 44 tanesi kavun (Cucumis melo Linn.), kalan 3 örnek ise acurdur (C. anguria Linn.). Enfekteli örnekler arasından, beş tanesi (üç tanesi kavun, iki tanesi acur) sekans analizleri için seçilmiştir. Gerçekleştirilen sekans analizleri sonucunda CmEV izolatları kendi aralarında nükleotit düzeyinde %93-99, amino asit düzeyinde ise %94-99 benzerlikler gösterdiği belirlenmiştir. Dünya izolatları ile yapılan benzerlik analizleri sonucunda ise nükleotit düzeyinde %93-98, amino asit düzeyinde ise %91-98 benzerlikler tespit edilmiştir. Gerçekleştirilen bu çalışma ile ülkemizde ilk kez CmEV enfeksiyonu kavun ve dünya için yeni bir konukçu kaydı olan acur bitkisinde tespit edilmiştir.

Project Number

FBA-2019-2873

References

  • 1. Baek, D., Lim, S. & Moon J.S. 2016. First report of Cucumis melo endornavirus on Cucumis melo var. makuwa in Korea. https://www.ncbi.nlm.nih.gov/nuccore/KX641269 (Date accessed: 20 December 2019)
  • 2. Çat, A., Yardımcı, N. & Kılıç, H.Ç. 2016. Identification of viral agents in the greenhouses in cucumber and pumpkin in Antalya province and counties. Süleyman Demirel University Journal of Natural and Applied Sciences, 20(1): 129-132.
  • 3. da Costa, A.C., Leal, E., Gill, D., de Pádua Milagres, F.A., Komninakis, S.V., Brustulin, R., Teles, M.A.R, Lobato, M.C.A.B.S., das Chagas, R.T., Abrão, M.F.N.S., Soares, C.V.D.A., Delwart, X.D.E., Sabino E.C. & Luchs, A. 2019. Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil. Virus Genes, 55(3): 332-338.
  • 4. Escalante, C., Sela, N., Dombrovsky, A. & Valverde, R. 2016. Interactions between bell pepper endornavirus, bell pepper, and acute plant viruses. In Proceedings of XVIth EUCARPIA Capsicum and Eggplant Working Group Meeting in Memoriam Dr. Alain Palloix, Kecskemét, Hungary, 231-237.
  • 5. Fukuhara, T., Moriyama, H., Pak, J.K., Hyakutake, T. & Nitta, T. 1993. Enigmatic double-stranded RNA in Japonica rice. Plant Molecular Biology, 21(6): 1121-1130.
  • 6. Fukuhara, T., Koga, R., Aoki, N., Yuki, C., Yamamoto, N., Oyama, N., Udagawa, T., Horiuchi, H., Miyazaki, S., Higashi, Y., Takeshita, M., Ikeda, K., Arakawa, M., Matsumoto, N. & Moriyama, H. 2006. The wide distribution of endornaviruses, large double-stranded RNA replicons with plasmid-like properties. Archives of Virology, 151(5): 995-1002.
  • 7. Fukuhara, T. 2019. Endornaviruses: persistent dsRNA viruses with symbiotic properties in diverse eukaryotes. Virus Genes, 55(2): 165-173.
  • 8. Herschlag, R., Escalante, C., de Souto, E.R., Khankhum, S., Okada, R. & Valverde, R.A. 2019. Occurrence of putative endornaviruses in non-cultivated plant species in South Louisiana. Archives of Virology, 164(7): 1863-1868.
  • 9. Jeffrey, C. 1980. A review of the Cucurbitaceae. Botanical Journal of the Linnean Society, 81(3): 233247.
  • 10. Kamberoglu, M.A., Caliskan, A.F. & Desbiez, C. 2016. Current status of some cucurbit viruses in Çukurova region (Adana and Mersin provinces) of Turkey and molecular characterization of zucchini yellow mosaic virus isolates. Romanian Biotechnological Letters, 21(4): 11709-11719.
  • 11. Kaya, A. & Erkan, S. 2011. Detection and incidence of viruses in cucurbits grown in Izmir, Aydın, Manisa and Balıkesir provinces. Plant Protection Bulletin, 51(4): 387-405.
  • 12. Keçe, M.A. & Kamberoğlu, M.A. 2016. Biological, serological and molecular detection of watermelon mosaic virus (WMV-2) in watermelon growing fields in Eastern Mediterranean region. Journal of Tekirdag Agricultural Faculty, 13(03): 156-164.
  • 13. Köklü, G. & Yilmaz, Ö. 2006. Occurrence of cucurbit viruses on field-grown melon and watermelon in the Thrace region of Turkey. Phytoprotection, 87(3): 123-130.
  • 14. Li, R., Mock, R., Huang, Q., Abad, J., Hartung, J. & Kinard, G. 2008. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. Journal of Virological Methods, 154(1-2): 48-55.
  • 15. Muhire, B.M., Varsani, A. & Martin, D.P. 2014. SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One, 9: 0108277.
  • 16. Okada, R., Kiyota, E., Sabanadzovic, S., Moriyama, H., Fukuhara, T., Saha, P., Roossinck, M.J., Severin, A. & Valverde, R.A. 2011. Bell pepper endornavirus: molecular and biological properties and occurrence in the genus Capsicum. Journal of General Virology, 92(11): 2664-2673.
  • 17. Ozaslan, M., Aytekin, T., Bas, B., Kilic, H., Afacan, I.D. & Dag, D.S. 2006. Virus diseases of cucurbits in Gaziantep, Turkey. The Plant Pathology Journal, 5(1): 24-27.
  • 18. Quito-Avila, D.F., Peralta, E.L., Martin, R.R., Ibarra, M.A., Alvarez, R.A., Mendoza, A., Insuasti, M. & Ochoa, J. 2014. Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region. European Journal of Plant Pathology, 140(2): 193-197.
  • 19. Sabanadzovic, S., Wintermantel, W.M., Valverde, R.A., McCreight, J.D. & Aboughanem-Sabanadzovic, N. 2016. Cucumis melo endornavirus: genome organization, host range and co-divergence with the host. Virus Research, 214: 49-58.
  • 20. Topkaya, S., Desbiez, C. & Ertunc, F. 2019. Presence of cucurbit viruses in Ankara and Antalya province and molecular characterization of coat protein gene of zucchini yellow mosaic virus Turkish isolates. Fresenius Environmental Bulletin, 28(4): 2442-2449.
  • 21. Valverde, R.A., Khalifa, M.E., Okada, R., Fukuhara, T., & Sabanadzovic, S. 2019. ICTV virus taxonomy profile: Endornaviridae. Journal of General Virology, 100(8): 1204-1205.
  • 22. Wakarchuk, D.A. & Hamilton, R.I. 1985. Cellular double-stranded RNA in Phaseolus vulgaris. Plant Molecular Biology, 5(1): 55-63.
  • 23. Zabalgogeazcoa, I.A. & Gildow, F.E. 1992. Double-stranded ribonucleic acid in ‘Barsoy’ barley. Plant Science, 83(2): 187-194.
  • 24. Zitter, T.A., Hopkins, D.L. & Thomas, C.E. 1996. Compendium of Cucurbit Diseases. The American Phytopathological Society Aps Press, St. Paulk, Minesota, 87p.
There are 24 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Research Article/Araştırma Makalesi
Authors

Ali Karanfil 0000-0002-4503-6344

Savaş Korkmaz 0000-0001-8227-3800

Project Number FBA-2019-2873
Publication Date April 15, 2020
Submission Date February 13, 2020
Acceptance Date April 11, 2020
Published in Issue Year 2020 Volume: 21 Issue: 1

Cite

APA Karanfil, A., & Korkmaz, S. (2020). FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY. Trakya University Journal of Natural Sciences, 21(1), 63-67. https://doi.org/10.23902/trkjnat.688646
AMA Karanfil A, Korkmaz S. FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY. Trakya Univ J Nat Sci. April 2020;21(1):63-67. doi:10.23902/trkjnat.688646
Chicago Karanfil, Ali, and Savaş Korkmaz. “FIRST REPORT OF Cucumis Melo Endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis Anguria Linn.), IN TURKEY”. Trakya University Journal of Natural Sciences 21, no. 1 (April 2020): 63-67. https://doi.org/10.23902/trkjnat.688646.
EndNote Karanfil A, Korkmaz S (April 1, 2020) FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY. Trakya University Journal of Natural Sciences 21 1 63–67.
IEEE A. Karanfil and S. Korkmaz, “FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY”, Trakya Univ J Nat Sci, vol. 21, no. 1, pp. 63–67, 2020, doi: 10.23902/trkjnat.688646.
ISNAD Karanfil, Ali - Korkmaz, Savaş. “FIRST REPORT OF Cucumis Melo Endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis Anguria Linn.), IN TURKEY”. Trakya University Journal of Natural Sciences 21/1 (April 2020), 63-67. https://doi.org/10.23902/trkjnat.688646.
JAMA Karanfil A, Korkmaz S. FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY. Trakya Univ J Nat Sci. 2020;21:63–67.
MLA Karanfil, Ali and Savaş Korkmaz. “FIRST REPORT OF Cucumis Melo Endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis Anguria Linn.), IN TURKEY”. Trakya University Journal of Natural Sciences, vol. 21, no. 1, 2020, pp. 63-67, doi:10.23902/trkjnat.688646.
Vancouver Karanfil A, Korkmaz S. FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY. Trakya Univ J Nat Sci. 2020;21(1):63-7.

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