DISTINCTIVE SIRE1 RETROTRANSPOSON PATTERNS ON BARLEY CHROMOSOMES?

Year 2021, Volume: 22 Issue: 1, 9 - 15, 15.04.2021

Elif Karlık , Nermin Gozukirmizi

https://doi.org/10.23902/trkjnat.773302

Abstract

SIRE1 is an active and relatively high copy-number retroelement belongs to the Tyl/Copia long terminal repeat (LTR) retrotransposon superfamily. Distinctive SIRE1 elements (ENV and GAG) distributions in barley genome were observed by using fluorescent in situ hybridization (FISH). We performed PCR to obtain tetramethylrhodamine-dUTP (TRITC)-labelled probes. Localizations of SIRE1 ENV and GAG domains were demonstrated under confocal microscope on Hordeum vulgare L. cv. Hasat root preparations. Our results revealed the distributions of SIRE1 elements ENV and GAG in barley genome. These results may provide to uncover the organization of SIRE retrotransposon pattern in barley genome.

Keywords

Fluorescence in situ hybridization, Retrotransposon, SIRE1, Barley, ENV, GAG

Thanks

The authors thank Dr. Stuart James Lucas for his kind revision.

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