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INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS

Year 2023, Volume: 24 Issue: 1, 11 - 20, 15.04.2023
https://doi.org/10.23902/trkjnat.1145462

Abstract

Pomegranate peel contains bioactive ingredients such as flavonoids, ellagitannins, phenolics and proanthocyanidin compounds with high antioxidant activity. Pomegranate peel has antiapoptotic, antioxidant and anti-inflammatory effects due to its high punicalagin content. We aimed to determine the effect of pomegranate peel extract (PPE) on lipopolysaccharide (LPS)-induced uveitis. Sixty rats were seperated randomly into twelve groups (n = 5). The healthy group received intraperitoneal normal saline, the uveitis group received 200 μg/kg LPS, the dexamethasone (DEX) group received 200 μg/kg LPS plus 1 mg/kg DEX, the PPE100, PPE300 and PPE500 groups received 200 μg/kg LPS plus 100, 300 and 500 mg/kg PPE, respectively. The eye tissues were collected at 3rd and 24th hour. and investigated molecularly (Relative quantification of gene expression), biochemically (Superoxide dismutase activity, Glutathione and Malondialdehyde levels) and histopathologically (staining with Harris Hematoxylin and Eosin Y). Tumor Necrosis Factor-α, vascular endothelial growth factor, and Caspase-3 levels markedly decreased in a dose-dependent manner in the uveitic rats following PPE administration. PPE administration significantly ameliorated uveitic disorders in oxidative stress factors including Glutathione, Superoxide dismutase and Malondialdehyde, with its effects raising in a dose-dependent manner. PPE eliminated histopathological changes in eye tissues due to uveitis. PPE can be a promising agent by contributing to alternative preventive treatment methods for uveitis with its anti-inflammatory, antioxidative, antiapoptotic and antiangiogenic effects.

Supporting Institution

the Scientific Research Council of Tekirdağ Namık Kemal University

Project Number

NKUBAP.02.GA.20.240

Thanks

Part of this study was presented orally at the 5th International Hippocrates Congress on Medical and Health Sciences on 18-19 December 2020.

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Year 2023, Volume: 24 Issue: 1, 11 - 20, 15.04.2023
https://doi.org/10.23902/trkjnat.1145462

Abstract

Nar kabuğu, yüksek antioksidan aktiviteye sahip flavonoitler, elajitanenler, fenolikler ve proantosiyanidin bileşikleri gibi biyoaktif bileşenler içermesi nedeniyle en faydalı parçalardan biridir. Nar kabuğundaki yüksek punikalagin içeriği, antienflamatuvar, antioksidan ve antiapoptotik etkiler üretmesini sağlar. Bu çalışmada deneysel lipopolisakkarit (LPS) kaynaklı üveit modelinde nar kabuğu ekstresinin (NKE) koruyucu etkilerini değerlendirmeyi amaçladık. 60 sıçan rastgele 12 gruba ayrıldı (n = 5). Sağlıklı grup intraperitoneal normal salin; üveit grubu 200 μg/kg LPS; deksametazon (DEX) grubu 200 μg/kg LPS ve 1 mg/kg DEX; NKE100, NKE300 ve NKE500 grupları ise 200 μg/kg LPS ile birlikte sırasıyla 100, 300 ve 500 mg/kg NKE aldı. Göz dokuları 3. ve 24. saatte toplandı ve moleküler, biyokimyasal ve histopatolojik olarak incelendi. NKE uygulamasını takiben üveitik sıçanlarda tümör nekroz faktörü-α, vasküler endotelyal büyüme faktörü ve kaspaz-3 seviyeleri doza bağlı olarak belirgin şekilde azaldı. NKE uygulaması, doza bağlı olarak artan etkileri ile Glutatyon, Süperoksit dismutaz ve Malondialdehit gibi oksidatif strese bağlı faktörlerdeki üveitik bozuklukları önemli ölçüde iyileştirdi. NKE ayrıca üveit ile ilişkili göz dokularındaki histopatolojik değişiklikleri de ortadan kaldırdı. NKE, antienflamatuvar, antioksidan, antiapoptotik ve antianjiyojenik etkileri ile üveit için alternatif koruyucu tedavi yöntemlerine katkıda bulunarak umut verici bir ajan olabilir.

Project Number

NKUBAP.02.GA.20.240

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There are 60 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Article/Araştırma Makalesi
Authors

Tuğba Nurcan Yüksel 0000-0001-5092-1674

Muhammed Yayla 0000-0002-0659-3084

Duygu Köse 0000-0002-3468-1567

Rüstem Anıl Uğan 0000-0002-4837-2343

Erdem Toktay 0000-0002-7447-6023

Pinar Aksu Kılıçle 0000-0002-3567-5775

Elif Çadırcı 0000-0003-0836-7205

Zekai Halıcı 0000-0001-6854-6059

Project Number NKUBAP.02.GA.20.240
Publication Date April 15, 2023
Submission Date July 19, 2022
Acceptance Date October 28, 2022
Published in Issue Year 2023 Volume: 24 Issue: 1

Cite

APA Yüksel, T. N., Yayla, M., Köse, D., Uğan, R. A., et al. (2023). INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS. Trakya University Journal of Natural Sciences, 24(1), 11-20. https://doi.org/10.23902/trkjnat.1145462
AMA Yüksel TN, Yayla M, Köse D, Uğan RA, Toktay E, Aksu Kılıçle P, Çadırcı E, Halıcı Z. INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS. Trakya Univ J Nat Sci. April 2023;24(1):11-20. doi:10.23902/trkjnat.1145462
Chicago Yüksel, Tuğba Nurcan, Muhammed Yayla, Duygu Köse, Rüstem Anıl Uğan, Erdem Toktay, Pinar Aksu Kılıçle, Elif Çadırcı, and Zekai Halıcı. “INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica Granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS”. Trakya University Journal of Natural Sciences 24, no. 1 (April 2023): 11-20. https://doi.org/10.23902/trkjnat.1145462.
EndNote Yüksel TN, Yayla M, Köse D, Uğan RA, Toktay E, Aksu Kılıçle P, Çadırcı E, Halıcı Z (April 1, 2023) INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS. Trakya University Journal of Natural Sciences 24 1 11–20.
IEEE T. N. Yüksel, “INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS”, Trakya Univ J Nat Sci, vol. 24, no. 1, pp. 11–20, 2023, doi: 10.23902/trkjnat.1145462.
ISNAD Yüksel, Tuğba Nurcan et al. “INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica Granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS”. Trakya University Journal of Natural Sciences 24/1 (April 2023), 11-20. https://doi.org/10.23902/trkjnat.1145462.
JAMA Yüksel TN, Yayla M, Köse D, Uğan RA, Toktay E, Aksu Kılıçle P, Çadırcı E, Halıcı Z. INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS. Trakya Univ J Nat Sci. 2023;24:11–20.
MLA Yüksel, Tuğba Nurcan et al. “INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica Granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS”. Trakya University Journal of Natural Sciences, vol. 24, no. 1, 2023, pp. 11-20, doi:10.23902/trkjnat.1145462.
Vancouver Yüksel TN, Yayla M, Köse D, Uğan RA, Toktay E, Aksu Kılıçle P, Çadırcı E, Halıcı Z. INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS. Trakya Univ J Nat Sci. 2023;24(1):11-20.

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