Investigation of the putative functional relevance of the IL-6 3'UTR genetic variants with athletic phenotype in Turkish triathletes

Year 2024, Issue: Online First

Dilek Pirim , Hasan Faruk Niş , Fatih Atilla Bağcı

https://doi.org/10.23902/trkjnat.1493225

Abstract

Previous research suggests that genetic variants in the interleukin-6 (IL-6) gene contribute to sport-related traits and athletic performance. We aimed to identify sequence variants in the IL-6 gene region comprising the 3' untranslated region (UTR) in the Turkish triathletes and sedentary individuals and assessed their putative roles in tendency to athletic phenotype. Sequence variants were identified in the Turkish triathletes (n = 47) and sedentary individu-als (n = 46) by Sanger sequencing. Allele/genotype frequencies and linkage disequilibrium (LD) patterns were calculated by the Haploview program. The functional significance of the detected variants was analyzed using in silico prediction tools. Four single nucleotide vari-ants (rs13306435, rs747302620, rs2069849, rs13306436) were detected in saliva samples of the participants by sequencing the target region. Notably, rs13306436-3'UTR/IL-6 was only seen in the triathletes, while the exonic rs747302620 was observed in only sedentary group. Also, rs13306436G>A causes loss/gain sites for binding multiple miRNAs that may be associated with athletic performance. Our findings indicate that the 3'UTR/IL-6 may have functional relevance in determining sports talent. Future comprehensive studies focusing on the IL-6 gene in athletes may pave the way for not only determining the athletic status of the individuals but also have implications for translational medicine.

Keywords

3'UTR, bioinformatics, SNV, sport genetics, Interleukin-6, Triathletes

Ethical Statement

Ethics committee approval was received for this study from the Institutional Review Board (IRB) of the Bursa Uludag University by the number 2022-1/18. All participants were informed about the study and participated after signing informed consent forms. Sample collections in the race areas were accomplished with the approval and permission of the Turkish Triathlon Federation.

Supporting Institution

Bursa Uludag University Scientific Research Unit

Project Number

FYL-2022-807

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