Year 2025,
Issue: Online First
Ayşenur Kalmer İpek
,
Sedat Kesici
,
Ayten Dizkırıcı
,
Yusuf Uzun
Abstract
Hebeloma turleri doğada geniş yayılım gosteren ektomikorizal mantarlardır ve cok ceşitli habitatlarda yetişirler. Hebeloma turleri, turler arasındaki yuksek morfolojik benzerlik nedeniyle tanımlanması ve ayırt edilmesi zordur. Bu calışmada, Turkiye’den iki Hebeloma (Fr.) P. Kumm. turu (H. minus Bruchet ve H. rostratum Beker, Vesterh. & U. Eberh.) tanımlanmış ve fotoğraflanmıştır. Ornekler, molekuler ve morfolojik yontemler kullanılarak belirlenmiştir. İc transkripsiyon aralayıcı (ITS) ve translasyon uzatma faktoru 1-alfa (TEF1-α) bolgelerinin filogenisi Maksimum Olasılık yontemi kullanılarak oluşturulmuştur. Sonuc olarak, molekuler veriler ve morfolojinin birleşimi, Hebeloma turlerinin taksonomik durumunu değerlendirmek icin sağlam bir yaklaşım sunmuştur. Turlerin ayrıntılı acıklamaları ve cizimleri verilmiştir.
References
-
Acar, I., Uzun, Y., Kalmer, A., Dizkirici, T.A. & Öğün, Y. 2021. A new record for Turkish mycobiota from Selim (Kars) district. The Journal of Fungus, 12(1): 65-70.
https://doi.org/10.30708.mantar.820871
Acar, I., Dizkirici, A., Kalmer, A. & Uzun, Y. 2022. Hebeloma bingolense, a new species in section Hebeloma (Basidiomycota) from Turkey. Nova Hedwigia, 114(1-2): 55-68.
https://doi.org/10.1127/nova_hedwigia/2022/0673
-
Acar, İ., Kalmer, A. & Dizkirici, A. 2024. Confirmation of Hebeloma salicicola (Basidiomycota) from Türkiye. Austrian Journal of Mycology, 31: 89-97.
-
Beker, H.J., Eberhardt, U. & Vesterholt, J. 2016. Fungi Europaei 14, Hebeloma (Fr.) P. Kumm. Edizioni Candusso, Italia, 1218 pp.
-
Cripps, C.L., Eberhardt, U., Schütz, N., Beker, H.J., Evenson, V.S. & Horak, E. 2019. The genus Hebeloma in the Rocky Mountain Alpine Zone. MycoKeys, 46: 1-54.
https://doi.org/10.3897/mycokeys.46.32823
-
Dizkirici, A., Acar, I., Kalmer, A. & Uzun, Y. 2019. Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey. Kastamonu University Journal of Forestry Faculty, 19(1): 1-10. https://doi.org/10.17475/kastorman.543375
-
Dizkirici, A., Kalmer, A. & Acar, I. 2022. New Turkish records of Hebeloma excedens and H. geminatum, and confirmation of H. celatum. Mycotaxon, 137: 123-134. https://doi.org/10.5248/137.123
-
Doyle, J.J. & Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15.
-
Eberhardt, U., Beker, H.J. & Vesterholt, J. 2015. Decrypting the Hebeloma crustuliniforme complex: European species of Hebeloma section Denudata subsection Denudata (Agaricales). Persoonia, 35: 101-147. https://doi.org/10.3767/003158515X687704
-
Eberhardt, U., Beker, H.J., Vesterholt, J. & Schütz, N. 2016. The taxonomy of the European species of Hebeloma section Denudata subsections Hiemalia, Echinospora subsect. nov. and Clepsydroida subsect. nov. and five new species. Fungal Biology, 120: 72-103. https://doi.org/10.1016/j.funbio.2015.09.014
-
Eberhardt, U., Schütz, N., Krause, C. & Beker, H.J. 2018. Hebelomina (Agaricales) revisited and abandoned. Plant Ecology and Evolution, 151(1): 96-109. https://doi.org/10.5091/plecevo.2018.1361
-
Eberhardt, U., Beker, H.J., Schütz, N., Pedersen, O.S., Sysouphanthong, P. & Læssøe, T. 2020. Adventurous cuisine in Laos: Hebeloma parvisporum, a new species in Hebeloma section Porphyrospora. Mycologia, 112(1): 172-184. https://doi.org/10.1080/00275514.2019.1680220
-
Eberhardt, U., Schütz, N., Beker, H.J., Lee, S.S. & Horak, E. 2021a. Hebeloma in the Malay Peninsula: Masquerading within Psathyrella. MycoKeys, 77: 117-141. https://doi.org/10.3897/mycokeys.77.57394
-
Eberhardt, U., Beker, H.J., Borgen, T., Knudsen, H., Schütz, N. & Elborne, S.A. 2021b. A survey of Hebeloma (Hymenogastraceae) in Greenland. MycoKeys, 79: 17-118. https://doi.org/10.3897/mycokeys.79.63363
-
Eberhardt, U., Schütz, N., Bartlett, P., Hosaka, K., Kasuya, T. & Beker, H.J. 2022a. Revisiting Hebeloma (Hymenogastraceae, Agaricales) in Japan: four species recombined into other genera but three new species discovered. Mycological Progress, 21: 447-472. https://doi.org/10.1007/s11557-021-01757-x.
-
Eberhardt, U., Kong, A., Montoya, A., Schütz, N., Bartlett, P. & Beker, H.J. 2022b. Not (only) poison pies – Hebeloma (Agaricales, Hymenogastraceae) in Mexico. MycoKeys, 90: 163-202. https://doi.org/10.3897/mycokeys.90.85267
-
Eberhardt, U., Schütz, N., Bartlett, P. & Beker, H.J. 2022c. 96 North American taxa sorted – Peck’s Hebeloma revisited. Mycologia, 114(2): 337-387. https://doi.org/10.1080/00275514.2021.2012063
-
Finch TV (2025). http://www.geospiza.com (Accessed on February 17, 2025).
-
Grilli, E., Beker, H.J., Eberhardt, U., Schütz, N., Leonardi, M. & Vizzini, A. 2016. Unexpected species diversity and contrasting evolutionary hypotheses in Hebeloma (Agaricales) sections Sinapizantia and Velutipes in Europe. Mycological Progress, 15, (5). https://doi.org/10.1007/s11557-015-1148-6
-
Guindon, S., Dufayard J.F., Lefort V., Anisimova M., Hordijk W. & Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59(3): 307-321. https://doi.org/10.1093/sysbio/syq010
-
Hoang, D.T. Chernomor, O. von Haeseler, A. Minh, B.Q. & Vinh, L.S. 2018. UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35: 518-522. https://doi.org/10.1093/molbev/msx281
-
Index Fungorum (2025). https://www.indexfungorum.org. (Accessed on May 12, 2025).
-
Kalyaanamoorthy, S. Minh, B.Q. Wong, T.K.F. von Haeseler, A. & Jermiin L.S. 2017. ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14: 587-589. https://doi.org/10.1038/nmeth.4285
-
Lambert, H., Acar, I., Dizkirici Tekpinar, A., Fortin, G., Ivanushkina, N., Jacob,s K., Lamoureux, Y., Landry, J., Lebeuf, R., Kacha-lkin, A., Kalmer, A., Moncalvo, J.M., Paul, A., Van der Merwe, B., Williams, J., Zervakis, G.I., Kochkina, G., Vainshtein, M., Dima, B., Dovana, F., Ferisin, G., Malysheva, E., Papp, V., Ševcˇíková, H., Krisai-Greilhuber, I. & Haelewaters, D. 2024. Fungal systematics and evolution: FUSE 10. Sydowia, 76: 297-365. https://doi.org/10.12905/0380.sydowia76-2024-0297
-
Maddison, W.P. & Maddison, D.R. 2021. Mesquite: a modular system for Evolutionary analysis. Version 3.7.0. http://www.mesquiteproject.org
-
National Center for Biotechnology Information (NCBI). (2025). Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/
-
Nguyen, L.T. Schmidt, H.A. von Haeseler, A. & Minh B.Q. 2015. IQ-TREE A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32: 268-274. https://doi.org/10.1093/molbev/msu300
-
Parolly, G. 2004. The high mountain vegetation of Turkey - a state-of-the-art report, including a first annotated conspectus of the major syntaxa. Turkish Journal of Botany, 28: 39-63.
-
Rambaut, A. 2016. FigTree. Tree figure drawing tool v.1.4.3. Available from: http://tree.bio.ed.ac.uk/
-
Rehner, S.A. & Buckley, E. 2005. A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia, 97: 84-89. https://doi.org/10.1080/15572536.2006.11832842
-
Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A. & Chen, W. 2012. Fungal barcoding consortium nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. PNAS, 109: 6241-6246. https://doi.org/10.1073/pnas.1117018109
-
Sesli, E., Asan, A., Selçuk, F., Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H. H., Erdoğdu, M., Ergül, C. C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Y., Uzun, Y. & Yoltaş, A. 2020. Türkiye mantarları listesi (The checklist of Fungi of Türkiye). Ali Nihat Gökyiğit Vakfı Yayını, İstanbul, 688 pp.
-
Solak, M. & Türkoğlu, A. 2022. Macrofungi of Turkey, Checklist, Volume-III. Kanyılmaz Matbaacılık, İzmir.
-
Tekpinar, A.D. & Kalmer, A. 2019. Utility of various molecular markers in fungal identification and phylogeny. Nova Hedwigia, 109(1-2): 187-224. https://doi.org/10.1127/nova_hedwigia/2019/0528
-
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673-4680. https://doi.org/10.1093/nar/22.22.4673
-
Vesterholt, J. 2005. The genus Hebeloma. (Fungi of Northern Europe 3), Danish Mycological Society, Copenhagen, 146 pp.
-
Wen, J. & Zimmer, E.A. 1996. Phylogeny and biogeography of Panax L. (the Ginseng genus, Araliaceae): inferences from ITS sequences of nuclear ribosomal DNA. Molecular Phylogenetics and Evolution, 6: 167-177. https://doi.org/10.1006/mpev.1996.0069
Identification and phylogenetic analysis of two newly recorded Hebeloma species (Basidiomycota) from Türkiye
Year 2025,
Issue: Online First
Ayşenur Kalmer İpek
,
Sedat Kesici
,
Ayten Dizkırıcı
,
Yusuf Uzun
Abstract
Hebeloma species are ectomycorrhizal fungi that are widely distributed in nature and grow in a wide variety of habitats. Hebeloma spp. are difficult to identify and distinguish due to high morphological similarity between species. In this study, two newly recorded Hebeloma (Fr.) P. Kumm. species (H. minus Bruchet and H. rostratum Beker, Vesterh. & U. Eberh.) from Türkiye were described and photographed. Specimens were identified using morphological and molecular methods. Phylogenies based on the internal transcribed spacer (ITS) and translation elongation factor 1-alpha (TEF1-α)
regions of the genome were constructed employing the Maximum-Likelihood method. In conclusion, a technique that combines morphological and molecular data provides a robust approach to
assessing the taxonomic status of Hebeloma spp. Descriptions and photographs of the species are also presented.
Ethical Statement
Since the article does not contain any
studies with human or animal subject, its approval to the ethics committee
was not required.
References
-
Acar, I., Uzun, Y., Kalmer, A., Dizkirici, T.A. & Öğün, Y. 2021. A new record for Turkish mycobiota from Selim (Kars) district. The Journal of Fungus, 12(1): 65-70.
https://doi.org/10.30708.mantar.820871
Acar, I., Dizkirici, A., Kalmer, A. & Uzun, Y. 2022. Hebeloma bingolense, a new species in section Hebeloma (Basidiomycota) from Turkey. Nova Hedwigia, 114(1-2): 55-68.
https://doi.org/10.1127/nova_hedwigia/2022/0673
-
Acar, İ., Kalmer, A. & Dizkirici, A. 2024. Confirmation of Hebeloma salicicola (Basidiomycota) from Türkiye. Austrian Journal of Mycology, 31: 89-97.
-
Beker, H.J., Eberhardt, U. & Vesterholt, J. 2016. Fungi Europaei 14, Hebeloma (Fr.) P. Kumm. Edizioni Candusso, Italia, 1218 pp.
-
Cripps, C.L., Eberhardt, U., Schütz, N., Beker, H.J., Evenson, V.S. & Horak, E. 2019. The genus Hebeloma in the Rocky Mountain Alpine Zone. MycoKeys, 46: 1-54.
https://doi.org/10.3897/mycokeys.46.32823
-
Dizkirici, A., Acar, I., Kalmer, A. & Uzun, Y. 2019. Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey. Kastamonu University Journal of Forestry Faculty, 19(1): 1-10. https://doi.org/10.17475/kastorman.543375
-
Dizkirici, A., Kalmer, A. & Acar, I. 2022. New Turkish records of Hebeloma excedens and H. geminatum, and confirmation of H. celatum. Mycotaxon, 137: 123-134. https://doi.org/10.5248/137.123
-
Doyle, J.J. & Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15.
-
Eberhardt, U., Beker, H.J. & Vesterholt, J. 2015. Decrypting the Hebeloma crustuliniforme complex: European species of Hebeloma section Denudata subsection Denudata (Agaricales). Persoonia, 35: 101-147. https://doi.org/10.3767/003158515X687704
-
Eberhardt, U., Beker, H.J., Vesterholt, J. & Schütz, N. 2016. The taxonomy of the European species of Hebeloma section Denudata subsections Hiemalia, Echinospora subsect. nov. and Clepsydroida subsect. nov. and five new species. Fungal Biology, 120: 72-103. https://doi.org/10.1016/j.funbio.2015.09.014
-
Eberhardt, U., Schütz, N., Krause, C. & Beker, H.J. 2018. Hebelomina (Agaricales) revisited and abandoned. Plant Ecology and Evolution, 151(1): 96-109. https://doi.org/10.5091/plecevo.2018.1361
-
Eberhardt, U., Beker, H.J., Schütz, N., Pedersen, O.S., Sysouphanthong, P. & Læssøe, T. 2020. Adventurous cuisine in Laos: Hebeloma parvisporum, a new species in Hebeloma section Porphyrospora. Mycologia, 112(1): 172-184. https://doi.org/10.1080/00275514.2019.1680220
-
Eberhardt, U., Schütz, N., Beker, H.J., Lee, S.S. & Horak, E. 2021a. Hebeloma in the Malay Peninsula: Masquerading within Psathyrella. MycoKeys, 77: 117-141. https://doi.org/10.3897/mycokeys.77.57394
-
Eberhardt, U., Beker, H.J., Borgen, T., Knudsen, H., Schütz, N. & Elborne, S.A. 2021b. A survey of Hebeloma (Hymenogastraceae) in Greenland. MycoKeys, 79: 17-118. https://doi.org/10.3897/mycokeys.79.63363
-
Eberhardt, U., Schütz, N., Bartlett, P., Hosaka, K., Kasuya, T. & Beker, H.J. 2022a. Revisiting Hebeloma (Hymenogastraceae, Agaricales) in Japan: four species recombined into other genera but three new species discovered. Mycological Progress, 21: 447-472. https://doi.org/10.1007/s11557-021-01757-x.
-
Eberhardt, U., Kong, A., Montoya, A., Schütz, N., Bartlett, P. & Beker, H.J. 2022b. Not (only) poison pies – Hebeloma (Agaricales, Hymenogastraceae) in Mexico. MycoKeys, 90: 163-202. https://doi.org/10.3897/mycokeys.90.85267
-
Eberhardt, U., Schütz, N., Bartlett, P. & Beker, H.J. 2022c. 96 North American taxa sorted – Peck’s Hebeloma revisited. Mycologia, 114(2): 337-387. https://doi.org/10.1080/00275514.2021.2012063
-
Finch TV (2025). http://www.geospiza.com (Accessed on February 17, 2025).
-
Grilli, E., Beker, H.J., Eberhardt, U., Schütz, N., Leonardi, M. & Vizzini, A. 2016. Unexpected species diversity and contrasting evolutionary hypotheses in Hebeloma (Agaricales) sections Sinapizantia and Velutipes in Europe. Mycological Progress, 15, (5). https://doi.org/10.1007/s11557-015-1148-6
-
Guindon, S., Dufayard J.F., Lefort V., Anisimova M., Hordijk W. & Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59(3): 307-321. https://doi.org/10.1093/sysbio/syq010
-
Hoang, D.T. Chernomor, O. von Haeseler, A. Minh, B.Q. & Vinh, L.S. 2018. UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35: 518-522. https://doi.org/10.1093/molbev/msx281
-
Index Fungorum (2025). https://www.indexfungorum.org. (Accessed on May 12, 2025).
-
Kalyaanamoorthy, S. Minh, B.Q. Wong, T.K.F. von Haeseler, A. & Jermiin L.S. 2017. ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14: 587-589. https://doi.org/10.1038/nmeth.4285
-
Lambert, H., Acar, I., Dizkirici Tekpinar, A., Fortin, G., Ivanushkina, N., Jacob,s K., Lamoureux, Y., Landry, J., Lebeuf, R., Kacha-lkin, A., Kalmer, A., Moncalvo, J.M., Paul, A., Van der Merwe, B., Williams, J., Zervakis, G.I., Kochkina, G., Vainshtein, M., Dima, B., Dovana, F., Ferisin, G., Malysheva, E., Papp, V., Ševcˇíková, H., Krisai-Greilhuber, I. & Haelewaters, D. 2024. Fungal systematics and evolution: FUSE 10. Sydowia, 76: 297-365. https://doi.org/10.12905/0380.sydowia76-2024-0297
-
Maddison, W.P. & Maddison, D.R. 2021. Mesquite: a modular system for Evolutionary analysis. Version 3.7.0. http://www.mesquiteproject.org
-
National Center for Biotechnology Information (NCBI). (2025). Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/
-
Nguyen, L.T. Schmidt, H.A. von Haeseler, A. & Minh B.Q. 2015. IQ-TREE A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32: 268-274. https://doi.org/10.1093/molbev/msu300
-
Parolly, G. 2004. The high mountain vegetation of Turkey - a state-of-the-art report, including a first annotated conspectus of the major syntaxa. Turkish Journal of Botany, 28: 39-63.
-
Rambaut, A. 2016. FigTree. Tree figure drawing tool v.1.4.3. Available from: http://tree.bio.ed.ac.uk/
-
Rehner, S.A. & Buckley, E. 2005. A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia, 97: 84-89. https://doi.org/10.1080/15572536.2006.11832842
-
Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A. & Chen, W. 2012. Fungal barcoding consortium nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. PNAS, 109: 6241-6246. https://doi.org/10.1073/pnas.1117018109
-
Sesli, E., Asan, A., Selçuk, F., Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H. H., Erdoğdu, M., Ergül, C. C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Y., Uzun, Y. & Yoltaş, A. 2020. Türkiye mantarları listesi (The checklist of Fungi of Türkiye). Ali Nihat Gökyiğit Vakfı Yayını, İstanbul, 688 pp.
-
Solak, M. & Türkoğlu, A. 2022. Macrofungi of Turkey, Checklist, Volume-III. Kanyılmaz Matbaacılık, İzmir.
-
Tekpinar, A.D. & Kalmer, A. 2019. Utility of various molecular markers in fungal identification and phylogeny. Nova Hedwigia, 109(1-2): 187-224. https://doi.org/10.1127/nova_hedwigia/2019/0528
-
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673-4680. https://doi.org/10.1093/nar/22.22.4673
-
Vesterholt, J. 2005. The genus Hebeloma. (Fungi of Northern Europe 3), Danish Mycological Society, Copenhagen, 146 pp.
-
Wen, J. & Zimmer, E.A. 1996. Phylogeny and biogeography of Panax L. (the Ginseng genus, Araliaceae): inferences from ITS sequences of nuclear ribosomal DNA. Molecular Phylogenetics and Evolution, 6: 167-177. https://doi.org/10.1006/mpev.1996.0069