Oxidant–antioxidant system alterations in LPS-induced sepsis: Modulatory effects of pregabalin

Abstract

Objective: This study aimed to investigate the effects of pregabalin (PRG) on the oxidant–antioxidant system in brain, pancreas, spleen, and testicular tissues using a lipopolysaccharide (LPS)-induced sepsis model. Methods: Rats were randomly assigned to three groups: control, LPS, and LPS+PRG. The control group received equivalent volumes of saline intraperitoneally (i.p.) and orally. Sepsis was induced in the LPS group by administering LPS (5 mg/kg, i.p.). In the LPS+PRG group, PRG was administered orally at a dose of 30 mg/kg 1 hour before LPS injection; subsequently, LPS (5 mg/kg, i.p.) was administered. All rats were sacrificed 6 hours after LPS administration. Malondialdehyde (MDA), catalase (CAT), and su-peroxide dismutase (SOD) levels were measured in brain, pancreas, spleen, and testicular tissues to eval-uate the oxidant–antioxidant status. Results: In brain tissue, no statistically significant differences in MDA, CAT, or SOD levels were ob-served between the control and LPS groups (p > 0.05). MDA levels were significantly decreased in the PRG-treated group compared with both the control (p < 0.001) and LPS (p = 0.029) groups. CAT levels were also significantly reduced in the PRG-treated group compared with the control group (p = 0.040), whereas no statistically significant difference was observed compared to the LPS group (p = 0.240). In contrast, SOD levels were significantly increased in the PRG-treated group compared with both the con-trol (p < 0.001) and LPS (p < 0.001) groups. No statistically significant differences were observed in pancreas, spleen, or testicular tissues. Conclusion: PRG may modulate the oxidant–antioxidant system in brain tissue during LPS-induced systemic inflammation. Further studies are required to clarify its effects on the pancreas, spleen, and testicular tissues.

Keywords

• Sepsis
• Pregabalin
• Oksidant-Antioksidant System

Supporting Institution

Süleyman Demirel University Scientific Research Projects Coordination Unit

Project Number

Project number: TM-2019-7407

Ethical Statement

Burdur Mehmet Akif Ersoy University Animal Experiments Local Ethics Committee (29.11.2023/1211)

Thanks

None

LPS kaynaklı sepsiste oksidan-antioksidan sistem değişiklikleri: Pregabalinin modülatör etkileri

Abstract

Amaç: Bu çalışmanın amacı, lipopolisakkarit (LPS) ile indüklenen sepsis modelinde pregabalinin (PRG) oksidan–antioksidan sistem üzerindeki etkilerini beyin, pankreas, dalak ve testis dokularında araştırmaktır. Yöntem: Sıçanlar rastgele kontrol, LPS ve LPS+PRG olmak üzere üç gruba ayrıldı. Kontrol grubuna in-traperitoneal (i.p.) ve oral yolla eşdeğer hacimlerde salin uygulandı. LPS grubunda sepsis, 5 mg/kg dozunda i.p. LPS uygulanarak oluşturuldu. LPS+PRG grubuna, LPS uygulamasından 1 saat önce oral yolla 30 mg/kg pregabalin verildi; bunu takiben 1 saat sonra 5 mg/kg dozunda i.p. LPS uygulandı. Tüm hayvanlar, LPS uygulamasından 6 saat sonra sakrifiye edildi. Oksidan–antioksidan durumun değer-lendirilmesi amacıyla beyin, pankreas, dalak ve testis dokularında malondialdehit (MDA), katalaz (CAT) ve süperoksit dismutaz (SOD) düzeyleri ölçüldü. Bulgular: Beyin dokusunda PRG uygulanan grupta MDA düzeylerinin kontrol (p < 0.001) ve LPS (p = 0.029) gruplarına kıyasla istatistiksel olarak anlamlı derecede azaldığı saptandı. Kontrol grubuna kıyasla PRG uygulanan grupta CAT düzeyleri de anlamlı ölçüde azalırken (p = 0.040), LPS grubuna kıyasla istatistiksel açıdan anlamlı bir fark gözlenmedi (p = 0.240). Buna karşılık, SOD düzeyleri PRG uygulanan grupta hem kontrol hem de LPS gruplarına kıyasla istatistiksel olarak anlamlı düzeyde arttı (her iki karşılaştırma için p < 0.001). Pankreas, dalak ve testis dokularında ise istatistiksel olarak anlam-lı bir farklılık saptanmadı. Sonuç: PRG, LPS ile indüklenen sistemik inflamasyon sırasında beyin dokusundaki oksidan-antioksidan sistemini modüle edebilir. Pankreas, dalak ve testis dokuları üzerindeki olası etkilerinin aydınlatılabilmesi için daha ileri çalışmalara ihtiyaç vardır.

Keywords

• Sepsis
• Pregabalin
• Oksidan-Antioksidan Sistem

Supporting Institution

Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

Project number: TM-2019-7407

Ethical Statement

Burdur Mehmet Akif Ersoy Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu (29.11.2023/1211)

Thanks

Yok

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