Arabidopsis thaliana'daki ath-mir156 Gen Ailesinin in Siliko Analizi

Year 2022, Volume: 3 Issue: 1, 25 - 35, 27.10.2022

Merve Balaban , Behcet İnal

Abstract

MikroRNA (miRNA), gen ekspresyonunun transkripsiyon sonrası düzenlenmesinde işlev gören küçük (~ 22 nükleotid) kodlamayan bir RNA molekülüdür. Önceki çalışmalar, miRNA’nın bitkilerin olumsuz koşullara karşı direnç kabiliyetinde önemli bir rol oynadığını göstermiştir. Bu araştırmada, önemli bir model organizma olan Arabidopsis thaliana’da miR156 ailesine dizilerin olgun, öncül ve promotor dizi karakterizasyonu yapılmıştır. Biyoinformatik yaklaşımı kullanarak, ath-miR156 gen promotörlerinde öngörülen cis düzenleyici elementleri ve aynı anda miR156 hedefli genleri tahmin edilmiştir. Sonuçlar, olgun ath-miR156 dizilerinde yüksek koruma olduğunu, ancak öncü dizilerde olmadığı görülmüştür. ath-miR156 gen promotörlerinde bulunan çeşitli cis düzenleyici elementlerin olduğu tespit edilmiştir.

Keywords

miR156, mikroRNA, kodlamayan RNA, Arabidopsis

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