The Effects of REM Sleep Deprivation on Skeletal Muscle Myostatin Levels in Rats

Abstract

Aim: Sleep is one of the crucial factors for muscle metabolism. It has been shown that sleep deprivation (SD) impairs muscle regeneration. Myostatin is expressed by skeletal muscle cells and limits muscle growth. The purpose of our study was to evaluate the effects of acute sleep deprivation on skeletal muscle myostatin levels in rats. Material and Methods: Twenty one male Wistar albino rats (200-250g) were randomly allocated in to three groups (n=7): Control group, Wide platform (WP) group and SD group. REM SD was induced by using the modified multiple platform method for 72 hours. The locomotor activity were evaluated among groups using open field test (OFT). Gastrocnemius and soleus muscle tissue were harvested and the levels of myostatin, malondialdehyde (MDA), glutathione (GSH) and glycogen was measured in the muscle tissues. Results: This study showed that acute SD caused an increase in myostatin (1161±39.55) and MDA levels (115.37±8.47) in the soleus muscle compared to the control group (respectively p=0.036 ve p=0.01). Compared with the other groups, the number of crossing square was increased in OFT (56.33±22.02) (p=0.001 ve p=0.044). GSH (4.86±0.26) and glycogen levels (5.13±0.21) in gastrocnemius muscle was decresead in SD group (respectively p=0.007 ve p=0.028). Conclusion: These data may represent that REM sleep deprivation affects muscle metabolism by changing myostatin levels and oxidative stress parameters in skeletal muscle to different extents in different muscle types.

Keywords

• acute REM sleep deprivation
• myostatin
• skeletal muscle

Supporting Institution

TÜBİTAK 2209-A

Project Number

1919B012112894

Sıçanlarda REM Uyku Yoksunluğunun İskelet Kası Myostatin Düzeylerine Etkisi

Abstract

Amaç: Uyku, kas metabolizması için önemli faktörlerden biridir. Uyku yoksunluğunun (UY) kas rejenerasyonunu bozduğu gösterilmiştir. Myostatin iskelet kası hücreleri tarafından eksprese edilir ve kas büyümesini sınırlandırır. Çalışmamızın amacı, sıçanlarda akut uyku yoksunluğunun iskelet kası myostatin düzeyleri üzerindeki etkilerini değerlendirmektir. Gereç ve Yöntemler: Yirmi bir erkek Wistar albino sıçan (200-250g) rastgele üç gruba (n=7) ayrılmıştır: Kontrol grubu, Geniş platform (GP) grubu ve UY grubu. REM UY, 72 saat boyunca modifiye çoklu platform yöntemi kullanılarak indüklenmiştir. Lökomotor aktivite gruplar arasında açık alan testi (OFT) kullanılarak değerlendirilmiştir. Gastroknemius ve soleus kas dokuları alınmış ve kas dokularında myostatin, malondialdehit (MDA), glutatyon (GSH) ve glikojen seviyeleri ölçülmüştür. Bulgular: Bu çalışma, akut UY’nin kontrol grubuna kıyasla soleus kasında myostatin (1161±39,55) ve MDA (115,37±8,47) seviyelerinde artışa neden olduğunu göstermiştir (sırasıyla p=0,036 ve p=0,01). OFT’de geçilen kare sayısı diğer gruplarla karşılaştırıldığında artmıştır (56,33±22,02) (p=0,001 ve p=0,044). Gastroknemius kasında GSH (4,86±0,26) ve glikojen seviyeleri (5,13±0,21) UY grubunda azalmıştır (sırasıyla p=0,007 ve p=0,028). Sonuç: Bu veriler, REM uyku yoksunluğunun iskelet kasındaki myostatin seviyelerini ve oksidatif stres parametrelerini farklı kas tiplerinde farklı miktarlarda değiştirerek kas metabolizmasını etkilediğini gösterebilir.

Keywords

• Akut REM uyku yoksunluğu
• miyostatin
• iskelet kası

Project Number

1919B012112894

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