Diyabetik Sıçanlarda Melatonin Uygulamasının Karaciğer, Böbrek, Mide, Pankreas ve Göz Dokularında Oksidatif Stres Üzerine Etkisi

Abstract

Amaç: Oksidatif stresin diyabetik komplikasyonların gelişiminde çok önemli rol oynadığı yaygın olarak kabul edilmektedir. Böylece koruyucu tedaviler hastalığın olası yan etkilerini hafifletebilir. Bu çalışmanın amacı melatonin tedavisinin diyabetik sıçanların karaciğer, böbrek, pankreas, mide ve retinal dokularındaki oksidan ve antioksidan durumuna etkisini incelemektir.

Gereç ve Yöntemler: Erkek Wistar albino cinsi sıçanlar 4 gruba ayrıldılar: Kontrol, kontrol+melatonin, diyabetik ve diyabet+melatonin uygulanan grup. Sıçanlarda intraperitoneal (i.p) olarak tek doz streptozotosin (STZ) (60 mg/kg) uygulaması ile diyabet oluşturulmuştur. Melatonin 10 mg/kg dozunda günde tek doz i.p olarak 30 gün boyunca uygulanmıştır. 30 günlük tedavinin sonunda indirgenmiş glutatyon (GSH) ve malondialdehid (MDA) düzeyleri karaciğer, böbrek, pankreas, mide ve retinada ölçülmüştür.

Bulgular: Sonuçlarımız diyabetik hayvanların dokularında MDA düzeylerinin arttığını ve GSH seviyelerinin azaldığını göstermiştir. Melatonin uygulaması karaciğer dışındaki dokularda MDA düzeylerini anlamlı olarak düşürmüştür. Melatonin tedavisi diyabet grubuna göre karşılaştırıldığında GSH düzeylerinde pankreas ve mide dışındaki dokularda artış bulunmuştur.

Sonuç: Bu çalışmada sunulan sonuçlar, diyabete, diyabetik komplikasyonların başlıca nedeni olarak kabul edilen oksidatif stresin eşlik ettiği ortak görüşüyle uyumludur. Sonuçlarımız melatoninin diyabette artan oksidatif stres üzerinde iyileştirici etkisi ile kullanılabileceğini göstermektedir.

Keywords

• Diabetes mellitüs
• Melatonin
• Oksidatif stres
• Glutatyon

The Effect of Melatonin Administration in Diabetic Rats on Oxidative Stress in Liver, Kidney, Stomach, Pancreas and Eye Tissues

Abstract

Aim: It is commonly accepted that oxidative stress plays a crucial role in the development of diabetic complication. Thus, preventive therapy can alleviate the possible side effects of the disease. The aim of the present study was to investigate the effect of melatonin administration on the oxidant and antioxidant system in the liver, kidney, pancreas, stomach and retinal tissues of diabetic rats.

Material and Methods: Male Wistar albino rats were randomly divided into 4 groups: control; control+Melatonin treatment; diabetic; diabetic+melatonin treatment. Rats were injected with a single intraperitoneal (i.p) injection of streptozotocin (STZ) (60 mg/kg) to induce diabetes mellitus. Melatonin (10 mg/kg) was given daily by i.p injection for 30 days. After 30 days of treatment, the contents of reduced glutathione (GSH) and malondialdehyde (MDA) in liver, kidney, pancreas, stomach and retina were assayed.

Results: Our results showed that diabetic rats exhibited significant decreases in GSH level and exhibited a high level of MDA in tissues. Melatonin treatment significantly reduced the elevated MDA level all tissues except liver tissue. GSH levels were found to increase in all tissues except pancreas and stomach after melatonin treatment compared diabetes group.

Conclusion: The results presented in this study are in agreement with the common view that diabetes is accompanied by oxidative stress, which is regarded as the main cause of diabetic complications. Our results suggests that melatonin could be used for its ameliorative effect against oxidative stress in diabetes

Keywords

• Diabetes mellitus
• Melatonin
• Oxidative stress
• Glutathione

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