Lectin Staining of Extensor Digitorum Longus Muscle Cell Membranes in Alloxan Diabetic Rats

Year 2017, Volume: 1 Issue: 3, 105 - 110, 03.12.2017

Nursel Gül , Suna Cebesoy Nesrin Özsoy

Abstract

Aim: Effect of alloxan-diabet on Extensor digitorum longus (EDL) skeletal muscle of rats were observed by lectin staining techniques in light microscope.

Material and Methods: After 30 days alloxan (dose of alloxan: 55 mg/kg) injected by intravenously, samples of muscles were obtained from control and diabetic rats. The muscle samples sections were cutted by cryostat microtome and stained by four biotinylated lectins [Wheat Germ Agglutinin (WGA), Pea Nut Agglutinin (PNA), Concavalin A (ConA), Griffonia simplicifolia I (GS)]. The lectins were fixed by avidin-peroxidase complex. All lectins were bounded to EDL muscle cell membranes of control and diabetic rats.

Results: GS and WGA lectins were strongly (+++) stained extensor digitorum muscle cell membranes of alloxan-diabetic rats. Not only cell membranes but also cytoplasmic miyofibrills of diabetic muscle cells were stained by GS lectin. PNA was moderate (++) stained diabetic muscle cell membranes. Con A was weakly (+) stained diabetic muscle cell membranes with respect to control cell membranes. According to our findings, alloxan-diabetes altered the molecular structure of glycoproteins in cell membranes of EDL skeletal muscles of rats.

Conclusion: We suggested that this study will contribute to diabetes research to show the damaging effects of diabetes on cell membranes.

Keywords

Extensor digitorum longus muscle cell membrane, Lectin stainning, Diabetes mellitus, Alloxan

Alloxan Diyabetli Sıçanlarda Extensor Digitorum Longus Kas Hücresi Membranlarının Lektin Boyaması

Abstract

Amaç: Alloksan diyabetin sıçanların Extensor Digitorum Longus (EDL) iskelet kasına etkisi lektin boyama tekniği ile ışık mikroskobunda incelendi.

Gereç ve Yöntemler: İntravenöz yoldan alloksan enjekte edildikten 30 gün sonra, kontrol ve diyabetik grubu sıçanlardan kas örnekleri alındı. Kas örneklerinden kryostatlı mikrotom ile kesitleri alındı ve dört biyotinli lektin [Wheat Germ Agglutinin (WGA), Pea Nut Agglutinin (PNA), Concavalin A (ConA), Griffonia simplicifolia (GS)] ile boyandı. Lektinler, avidin-peroksidaz kompleksi ile tespit edildi. Lektinlerin hepsi kontrol ve diyabetik sıçan grubunun EDL iskelet kasına bağlandı.

Bulgular: GS ve WGA lektinleri alloksan diyabetik sıçanların EDL iskelet kaslarını kuvvetli (+++) boyadı. Özellikle GS lektini sadece hücre zarına değil aynı zamanda kas fibrillerini kuvvetli boyadı. PNA lektini orta derecede (++) EDL kaslarını boyadı. Con A ise kontrol grubuna göre diyabetik kasları daha zayıf (+) boyadı. Elde ettiğimiz bulgulara göre alloksan-diyabet sıçanların EDL iskelet kaslarının hücre zarlarındaki glikoproteinlerin moleküler yapısını değiştirmiştir.

Sonuç: Bu çalışma diyabetin hücre zarlarına hasar verici etkisini göstermek açısından diyabetle yapılan araştırmalara katkıda bulunacaktır.

Keywords

Extensor digitorum longus iskelet kası hücre zarı, Lektin boyama, Diabetes mellitus, Alloksan

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