Valorization of banana pseudostem: endoxylanase production by Streptomyces sp. SH5027 using statistical approaches and its characterization and application in bread making

Year 2022, Volume: 6 Issue: 2, 128 - 139, 15.04.2022

Ali Osman Adıgüzel

https://doi.org/10.31127/tuje.847736

Abstract

The present study aimed to achieve a cost-effective production of endoxylanase by Streptomyces sp. SH5027 using banana pseudostem with the combination of conventional and statistical optimization and to determine the biochemical properties of the enzyme and its effect on bread making. Enzyme production increased from 7.25 U/mL to 50.21 U/mL as a result of the optimization studies. The enzyme was stable at 50-75 °C and also retained more than 50% of its activity at pH 5.0-9.0 for an hour at optimum temperature. The calculated Km value for the purified enzyme was 1.689 mg/mL.min, while the Vmax value was 23.17 µmol/min.mg. The specific volume of the bread increased 9.6%, 12.8%, and 16.8% when 200 U, 300 U, and 400 U endoxylanase was added to the flour per kg, respectively. This study is the first to be conducted on the statistical optimization of endoxylanase production using banana pseudostem.

Keywords

Banana pseudostem, Endoxylanase, Streptomyces, RSM, Bread making

References

• Adıgüzel A O (2020). Production and characterization of thermo-, halo-and solvent-stable esterase from Bacillus mojavensis TH309. Biocatalysis and Biotransformation, 38(3), 210-226.
• Adigüzel A O & Tunçer M (2016). Production, characterization and application of a xylanase from Streptomyces sp. AOA40 in fruit juice and bakery industries. Food Biotechnol, 30(3), 189-218.
• Adıgüzel A O & Tunçer M (2017a). Production, purification, characterization and usage of a detergent additive of endoglucanase from isolated halotolerant Amycolatopsis cihanbeyliensis mutated strain Mut43. Biocatalysis and Biotransformation, 35(3), 197-204.
• Adıgüzel A O & Tunçer M (2017b). Production and characterization of partially purified thermostable endoxylanase and endoglucanase from novel Actinomadura geliboluensis and the biotechnological applications in the saccharification of lignocellulosic biomass. BioResources, 12(2), 2528-2547.
• Ahamed A & Vermette P (2008). Culture-based strategies to enhance cellulase enzyme production from Trichoderma reesei RUT-C30 in bioreactor culture conditions. Biochem Eng J, 40(3), 399-407.
• Alvindia D G & Natsuaki K T (2009). Biocontrol activities of Bacillus amyloliquefaciens DGA14 isolated from banana fruit surface against banana crown rot-causing pathogens. Crop Prot, 28(3), 236-242.
• Bradford M M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the, principle of protein-dye binding. Anal Biochem, 72, 248-254.
• Carvalho E A, dos Santos Góes L M, Uetanabaro A P T, da Silva E G P, Rodrigues L B, Pirovani C P & da Costa A M (2017). Thermoresistant xylanases from Trichoderma stromaticum: Application in bread making and manufacturing xylo-oligosaccharides. Food Chem, 221, 1499-1506.
• Courtin C M & Delcour J A (2002). Arabinoxylans and endoxylanases in wheat flour bread-making. J Cereal Sci, 35(3), 225-243.
• Food and Agriculture Organization. (2017). Banana statistical compendium. FAO, http://www.fao.org/fileadmin/templates/est/COMM_MARKETS_MONITORING/Bananas/Documents/Banana_Statistical_Compendium_2017.pdf
• Gabhane J, William S P, Gadhe A, Rath R, Vaidya A N & Wate S (2014). Pretreatment of banana agricultural waste for bio-ethanol production: Individual and interactive effects of acid and alkali pretreatments with autoclaving, microwave heating and ultrasonication. Waste Manage, 34(2), 498-503.
• Gilead S & Shoham Y (1995). Purification and characterization of alpha-L-arabinofuranosidase from Bacillus stearothermophilus T-6. Appl Environ Microbiol, 61(1), 170-174.
• Houfani A A, Větrovský T, Baldrian P & Benallaoua S (2017). Efficient screening of potential cellulases and hemicellulases produced by Bosea sp. FBZP-16 using the combination of enzyme assays and genome analysis. World J Microbiol Biotechnol, 33(29), 1-14.
• Kui H, Luo H, Shi P, Bai Y, Yuan T, Wang Y, Yang P, Dong S & Yao B (2010). Gene cloning, expression, and characterization of a thermostable xylanase from Nesterenkonia xinjiangensis CCTCC AA001025. Appl Biochem Biotechnol, 162(4), 953-965.
• Kumar A, Gupta R, Shrivastava B, Khasa Y P & Kuhad R C (2012). Xylanase production from an alkalophilic actinomycete isolate Streptomyces sp. RCK-2010, its characterization and application in saccharification of second generation biomass. J Mol Catal B: Enzym, 74(3-4), 170-177.
• Kumar V & Satyanarayana T (2012). Thermo-alkali-stable xylanase of a novel polyextremophilic Bacillus halodurans TSEV1 and its application in biobleaching. Int Biodeterior Biodegradation 75:138-145.
• Kumar V & Shukla P (2018). Extracellular xylanase production from T. lanuginosus VAPS24 at pilot scale and thermostability enhancement by immobilization. Process Biochem, 71, 53-60.
• Li N, Meng K, Wang Y, Shi P, Luo H, Bai Y, Yang P & Yao B (2008). Cloning, expression, and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp. S9. Appl Microbiol Biotechnol, 80(2), 231.
• Li Q, Sun B, Li X, Xiong K, Xu Y, Yang R, Hou J & Teng C (2018). Improvement of the catalytic characteristics of a salt-tolerant GH10 xylanase from Streptomyces rochei L10904. Int J Biol Macromol, 107, 1447-1455.
• Li X, Li E, Zhu Y, Teng C, Sun B, Song H & Yang R (2012). A typical endo-xylanase from Streptomyces rameus L2001 and its unique characteristics in xylooligosaccharide production. Carbohydr Res, 359, 30-36.
• Liu Z, Zhao X & Bai F (2013). Production of xylanase by an alkaline-tolerant marine-derived Streptomyces viridochromogenes strain and improvement by ribosome engineering. Appl Microbiol Biotechnol, 97(10), 4361-4368.
• Maheswari M U & Chandra T S (2000). Production and potential applications of a xylanase from a new strain of Streptomyces cuspidosporus. World J Microbiol Biotechnol, 16(3), 257-263.
• Mander P, Choi Y H, Pradeep G C, Choi Y S, Hong J H, Cho S & Yoo J C (2014). Biochemical characterization of xylanase produced from Streptomyces sp. CS624 using an agro residue substrate. Process Biochem 49(3), 451-456.
• Miller GL (1959) Use of dinitrosalisylic acid reagent for determination of reducing sugar. Anal Chem, 31, 426-428.
• Nascimento R P, Coelho R R R, Marques S, Alves L, Gırio F M, Bon E P S & Amaral-Collaço M T (2002). Production and partial characterisation of xylanase from Streptomyces sp. strain AMT-3 isolated from Brazilian cerrado soil. Enzyme Microb Technol, 31(4), 549-555.
• Ninawe S, Kapoor M & Kuhad R C (2008). Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32. Bioresour Technol, 99(5), 1252-1258.
• Padam B S, Tin H S, Chye F Y & Abdullah M I (2014). Banana by-products: an under-utilized renewable food biomass with great potential. J Food Sci Technol, 51(12), 3527-3545.
• Pennacchio A, Ventorino V, Cimini D, Pepe O, Schiraldi C, Inverso M & Faraco V (2018). Isolation of new cellulase and xylanase producing strains and application to lignocellulosic biomasses hydrolysis and succinic acid production. Bioresour Technol 259, 325-333.
• Porsuk I, Özakın S, Bali B & Yilmaz E I (2013). A cellulase-free, thermoactive, and alkali xylanase production by terrestrial Streptomyces sp. CA24. Turkish J Biol 37(3):370-375.
• Pradeep G C, Choi Y H, Choi Y S, Seong C N, Cho S S, Lee H J & Yoo J C (2013). A novel thermostable cellulase free xylanase stable in broad range of pH from Streptomyces sp. CS428. Process Biochem, 48(8), 1188-1196.
• Qiu Z, Shi P, Luo H, Bai Y, Yuan T, Yang P, Liu S & Yao B (2010). A xylanase with broad pH and temperature adaptability from Streptomyces megasporus DSM 41476, and its potential application in brewing industry. Enzyme Microb Technol, 46(6), 506-512.
• Sanjivkumar M, Silambarasan T, Palavesam A, Immanuel G (2017). Biosynthesis, purification and characterization of β-1, 4-xylanase from a novel mangrove associated actinobacterium Streptomyces olivaceus (MSU3) and its applications. Protein Expr Purif, 130, 1-12.
• Shah M P, Reddy G V, Banerjee R, Babu P R & Kothari I L (2005). Microbial degradation of banana waste under solid state bioprocessing using two lignocellulolytic fungi (Phylosticta spp. MPS-001 and Aspergillus spp. MPS-002). Process Biochem, 40(1), 445-451.
• Sharma S & Bajaj B K (2018). Xylanase production from a new strain of Aspergillus terreus S9 and its application for saccharification of rice straw using combinatorial approach. Environ Prog Sustain, 37(3), 1210-1219.
• Shin J H, Choi J H, Lee O S, Kim Y M, Lee D S, Kwak Y Y, Kim W C & Rhee I K (2009). Thermostable xylanase from Streptomyces thermocyaneoviolaceus for optimal production of xylooligosaccharides. Biotechnol Bioprocess Eng, 14(4), 391-399.
• Su Y, Zhang X, Hou Z, Zhu X, Guo X & Ling P (2011). Improvement of xylanase production by thermophilic fungus Thermomyces lanuginosus SDYKY-1 using response surface methodology. N Biotechnol, 28(1), 40-46.
• Thomas L, Sindhu R & Pandey A (2013). Identification and characterization of a highly alkaline and thermotolerant novel xylanase from Streptomyces sp. Biologia, 68(6), 1022-1027.
• Vieira W B, Moreira L R S, Neto A M & Filh E X F (2007). Production and characterization of an enzyme complex from a new strain of Clostridium thermocellum with emphasis on its xylanase activity. Braz J Microbiol, 38, 237-242.
• Walia A, Guleria S, Mehta P, Chauhan A & Parkash J (2017). Microbial xylanases and their industrial application in pulp and paper biobleaching: a review. 3 Biotech, 7(1), 11.
• Wang Y, Zhang H, He Y, Luo H & Yao B (2007). Characterization, gene cloning, and expression of a novel xylanase XYNB from Streptomyces olivaceoviridis A1. Aquaculture 1(267), 328-334.
• Yabalak E, Adigüzel S K, Adıgüzel A O, Ergene R S, Tuncer, M & Gizir A M (2017). Application of response surface methodology for the optimization of oxacillin degradation by subcritical water oxidation using H2O2: genotoxicity and antimicrobial activity analysis of treated samples. Desalination and Water Treatment, 81, 186-198.
• Yan Q, Hao S, Jiang Z, Zhai Q & Chen W (2009). Properties of a xylanase from Streptomyces matensis being suitable for xylooligosaccharides production. J Mol Catal B: Enzym, 58(1-4), 72-77.
• Zhou J, Shi P, Zhang R, Huang H, Meng K, Yang P & Yao B (2011). Symbiotic Streptomyces sp. TN119 GH 11 xylanase: a new pH-stable, protease-and SDS-resistant xylanase. J. Ind. Microbiol Biotechnol, 38(4), 523-530.