EXPRESSIONS OF APOPTOSIS-RELATED PROTEINS IN COLON CANCER CELLS AFTER HYP-INDUCED PHOTOTHERAPY
Abstract
Coloractal
cancer is the third most common cancer and is prominent death factor worlwide.
Although the main treatment is a surgery, the alternative suggested treatment
method is photodynamic therapy (PDT). Hypericin (HYP) is a well-studied
photosensitizier for PDT, however mechanism of action is still unclear. The aim
of the present study is to investigate the apoptotic cell mechanism in HT-29
and Caco-2 cells photoactivated with HYP.
In the present
study, HT-29 (grade-I) and Caco-2 (grade-II) human colon cancer cells treated
with 0.04, 0.08 or 0.15 µM HYP and irradiated with (4J/cm2) flourescent lamps.
HYP effects were evaluated for 16 and 24 h. Total protein content of cells were
measured by Bradford assay. Apoptosis-related proteins including caspase-9,
Bcl-2 and Bax expressions were investigated at the mRNA level by real-time PCR
for both cells.
Protein
concentrationdramatically decreasedin all groups HT-29 cellsafter 24 h and
Caco-2 cells. However, it increased in 0.15 µM group in Caco-2 cells. Caspase-9
were upregulated for both cells in 24 h incubation. Bcl-2 expression was not
observed in HT-29 cells, however Bcl-2 downregulated in Caco-2 cells. Bax
expression downregulated in HT-29 cells after 24 h incubation and upregulated
in Caco-2 cells after 16 h incubation.
In conclusion,
Caco-2 and HT-29 colon cancer cells response distinctly to HYP-mediated PDT in
terms of apoptosis-related proteins, activation of repair mechanisms and
resistance development.
This research was partially supported by the
Hacettepe University Scientific Research Projects Coordination Unit (09 01 601
011).
Keywords
References
- Aysun Kılıç SÜLOĞLU, Elif KARACAOĞLU, Güldeniz SELMANOĞLU Çağatay KARAASLAN
- Hacettepe University, Faculty of Science, Department of Biology, Zoology Section, Beytepe, Ankara, Turkey Hacettepe University, Faculty of Science, Department of Biology, Molecular Biology Section, Beytepe, Ankara, Turkey
Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
February 16, 2017
Submission Date
April 14, 2017
Acceptance Date
-
Published in Issue
Year 2017 Volume: Volume 2 Number: İssue 1 (1)