FOR RATS WHICH ARE IMPLEMENTED CADMIUM CHLORIDE EXPOSURE, POLYDATIN AND GRAPE SEED EXTRACTS PROTECTIVE EFFECTS ON TESTIS AND BRAIN TISSUES

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 20 - 20, 16.02.2017

Mustafa Evcimen Hasan Huseyin Demirel Recep Aslan Mehmet Sukru Gulay

Abstract

This study is carried out on rats, the polydatin
(PD) and grape seed extracts’ (GSE) protective effects aganist to cadmium’s
(Cd) oxidative damage in brain and testis (transgressing blood-testis and
blood-brain barriers) is investigated. In our study, 49 wistar-Albino adult
male rats were used. Rats were seperated into seven equal groups: Group 1;
Control Group (Normal saline was given orally for 30 days.) Group 2; Cd group
(5 mg/kg dose of CdCI₂ was dissolved in normal saline and was given
orally for 30 days.) Group 3; Group PD (g 120 mg/kg PD was dissolved in normal
saline and was given orally for 30 days.) Group 4; GSE Group (120 mg/kg GSE was
dissolved in normal saline and was given orally for 30 days.) Group 5; Cd+PD
Group (5 mg/kg dose of CdCI₂ + 120 mg/kg PD were dissolved in normal
saline and was given orally for 30 days.) Group 6; Cd+ GSE Group (5 mg/kg dose
of CdCI₂ + 120 mg were dissolved in normal saline and were given
orally for 30 days.) Group 7; Cd+PD+ GSE Group (5 mg/kg dose of CdCI₂+120
mg/kg PD + 120 mg/kg GSE were dissolved in normal saline and were given orally
for 30 days.) At the end of the experimental period, all the rats were decapitated
and their brain and testis tissues samples were examined. In Cd implemented
rats, in their brain (p <0.05) and in testis (p<0.01), malondialdehyde
(MDA) levels statistically increase in significant way, within PD and GSE
implemented groups, it decreases /kg GSE was detected.  In Cd implemented rats, antioxidant potential
values (AOP) decrease in brain and testis, in PD and GSE implemented groups, it
increases significantly. (p<0.001) According to the ICP-OES data, it
couldn’t be determined because it was under the brain measurement leels, in Cd
impelemented testis groups, there was increasement (p<0.001). Consequently,
for rats, because of the cadmium exposure, oxidative damage has occured in
brain and testis; aganist to this damage, both PD and GSE were protective, but
PD was more protective than GSE.

Keywords

FOR RATS WHICH ARE IMPLEMENTED CADMIUM CHLORIDE EXPOSURE

References

• Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Physiology, Afyonkarahisar, Turkey
• Afyon Kocatepe University, Bayat Vocational School, Afyonkarahisar, Turkey
• Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Physiology, Burdur, Turkey