Abstract
References
- Department of Biochemistry, Yıldırım Beyazıt University, Ankara, Turkey
- Department of Internal Medicine, Hacettepe University, Ankara, Turkey
- Department of Family Medicine, Occupational Diseases Hospital, Ankara, Turkey
- Department of Biochemistry, Atatürk Educational and Research Hospital, Ankara, Turkey
- Department of Toxicology, Occupational Diseases Hospital, Ankara, Turkey
Abstract
Styrene is the predominant
solvent used in printer. It is a colorless and volatile aromatic hydrocarbon
with an original smell. Main accused toxic substance for styrene induced
oxidative stress is styrene-7,8-oxide. Phenylglyoxylic acid (PGA) and mandelic
acid (MA) are the basic metabolites that are used for sytrene exposure
screening. In this study we aimed to investigate the association between
styrene metabolites and dynamic disulfide/thiol
homeostasis in people working in a printing center.
30 non-smoker, inkjet printing workers, included as the
study subjects. 30 non-smokers with no exposure were recruited as the control
group. The study was approved by the local ethic committee. We used Erel and Neşelioğlu’s method for serum disulfide/thiol homeostasis
measurements. PGA, MA, hippuric acid, methyl hippuric acid levels were detected by
high-performance liquid chromatography using
same commercial kit. Phenol levels detected with a commercial kit (Eureka, Italy), too
The
urinary PGA+MA, hippuric acids and phenol values of worker group were
significantly higher than the control group (p<0.001, p=0.001 and p=0.001,
respectively). There wasn’t any significant difference in terms of methyl hippuric acid between groups (p=0.101). Serum
disulfide levels, disulfide /native thiol ratios and
disulfide/total thiol ratios of exposed group were significantly higher than
control group while native thiol/total thiol ratio was significantly lower
(p<0.001).
With this study we, for
the first time demonstrate that the breakdown of tiol-disulfiide balance in
human body by styrene exposure.
Disulfide/thiol homeostasis measurements can be a
useful and reliable marker for oxidative stress caused by styrene exposure.
References
- Department of Biochemistry, Yıldırım Beyazıt University, Ankara, Turkey
- Department of Internal Medicine, Hacettepe University, Ankara, Turkey
- Department of Family Medicine, Occupational Diseases Hospital, Ankara, Turkey
- Department of Biochemistry, Atatürk Educational and Research Hospital, Ankara, Turkey
- Department of Toxicology, Occupational Diseases Hospital, Ankara, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
