DYNAMIC THIOL-DISULFIDE HOMEOSTASIS IN MERCURY-EXPOSED PATIENTS

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 51 - 51, 16.02.2017

Salim Neşelioğlu , Murat Alışık Ceylan Bal Almila Şenat Özcan Erel

Abstract

We aimed to
examine dynamic thiol-disulfide homeostasis (TDH) with a novel and automated
method in mercury-exposed patients (MEP). Mercury is the unique
metal which is liquid at room temperature and can readily evaporate. It uses
lots of industries such as battery, thermometer and dye manufacturing. Mercury
disrupts the activity of enzymes by binding to thiol groups and leads to cell
death. Compounds containing thiol groups can chelate with mercury. These
compounds can be used as an antidote because of these features in mercury
poisoning. Thiols, also known as mercaptans are organic compounds that
linked to a carbon atom of a sulfhydryl group. Thiol groups and disulphide
bonds can be convert to each other by oxidation-reduction reactions in plasma.
Thus, dynamic TDH is maintained. TDH has critical role in detoxification,
antioxidant protection, apoptosis and regulation of enzyme activities.

Plasma
native thiol and total thiol concentrations were determined with a novel
and automated measurement method. Half of subtract of
concentration of total thiol and native thiol is gave disulphide amount. Urine
mercury levels was measured by atomic absorption spectrometry.

Native
thiol and total thiol levels were not different in MEP comparing to control
group. But disulphide levels were increased
significantly (p=0.028). Native thiol, total thiol and
disulphide levels were significantly correlated with urine mercury levels
(respectively; p=0.012;r=-0.46,p<0.001;r=-0.58,p=0.007;r =-0.49)

TDH system
shifted to the side of disulphide bond formation in Mercury-exposed patients.
The novel test we used in this study may be useful for evaluating the
oxidative status in mercury-exposure.

Keywords

DYNAMIC THIOL-DISULFIDE HOMEOSTASIS IN MERCURY-EXPOSED PATIENTS

References

• Yildirim Beyazit University Clinical Biochemistry, Ankara, Turkey