DIAGNOSIS OF MESOTHELIOMA FROM HUMAN BLOOD SERUM VIA INFRARED SPECTROSCOPY COUPLED WITH CHEMOMETRICS

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 96 - 96, 16.02.2017

Dilek Yonar Salih Emrı Feride Severcan

Abstract

Malignant pleural mesothelioma (MPM) is an aggressive
and rare cancer that develops on the lining of the lungs called pleura. The
disease is primarily caused by the inhalation of microscopic asbestos fibers.
Mesothelioma can only be diagnosed in the advanced stage, because early
symptoms of it are often mistaken as other respiratory diseases. Hence, the
present study aimed to develop a rapid and operator independent method
with high specificity and sensitivity for MPM diagnosis from human blood serum
by Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR)
spectroscopy and chemometrics. FTIR spectra of the samples collected from
patients diagnosed with malignant pleural
mesothelioma (MPM), lung cancer (LC), and healthy control (C) were
recorded and analyzed with Perkin Elmer Spectrum program. Spectral analysis
indicated significant differences in lipid structure and function, such as
lipid order and fluidity respectively, protein, nucleic acid and glycogen
content of MPM patients. Both unsupervised (Principal Component Analysis, PCA
and Hierarchical Cluster Analysis, HCA) and supervised (Soft Independent
Modeling by Class Analogy, SIMCA) chemometric analyses were applied to classify
MPM patients based on the spectral differences. PCA results revealed an obvious
clustering of C, LC and MPM groups. Successful differentiations with 71-94%
sensitivity and 80-94% specifity were obtained from serum by HCA. Moreover, 83% accurate classification of groups was
obtained with supervised SIMCA method. This study demonstrated that ATR-FTIR spectroscopy coupled with chemometrics has great potential to diagnose MPM disease with higher sensitivity, specificity and
accuracy. 

 

*This work was
supported by the Scientific and Technical Research Council of Turkey (TUBITAK),
SBAG-113S294 Research Fund.

Keywords

DIAGNOSIS OF MESOTHELIOMA FROM HUMAN BLOOD SERUM VIA INFRARED SPECTROSCOPY COUPLED WITH CHEMOMETRICS

References

• Dilek YONAR, Salih EMRI, Feride SEVERCAN 1Middle East Technical University, Department of Biological Sciences, Ankara, Turkey 2Hacettepe University, Department of Chest Diseases, Ankara, Turkey