THE GENOTOXICITY AND CYTOTOXICITY OF FUNGUCITE (LUNA EXPERIENCE-EC 400) FLUOPYRAM/TEBACONAZOL COMBINATION SINGLE GEL CELL ELECTROPHORESIS (COMET ASSAY) AND MICRONUCLEUS ASSAY IN RAT BONE MARROW
Abstract
Pesticides are
chemical substances that prevent from harmful organisms and reduce their harms.
Pesticides areclassified by their durability time, chemical structure and
organisms which it affected. Fungicide is a general term thatgiven to all
chemicals which are used to take under control the fungus that infect on
plants. Luna Experience SC-400 is a new generation fungicide that combinated
with Fluopyram and Tebuconazole. Tebuconazole is a triazole fungicide. Tebuconazole
was tested in vitro for its potential genotoxic and cytotoxic effects on
cultured bovine peripheral lymphocytes. Fluopyram is a benzamide fungicide.
Genotoxic effects of fluopyram was examined and reported in rats. In this
study, We used three different doses (5 µg/kg, 10 µg/kg, 20 µg/kg) of Luna
Experience-EC 400 during 30 day at 48 h intervals by gavage in rats. We
investigated the genotoxicty and cytotoxicty of Luna Experience-EC 400 using
comet assay and micronucleus test in rat bone marrow. We found that Luna
Experience-EC 400 has genotoxic and cytotoxic potential in rat bone marrow. There is significant difference between control
group and all the doses of Luna Experience-EC 400 (p<0.05) for comet assay
levels and micronucleus frequency. In this study, we also investigated the
PCE/total erythrocyte rate. All the doses of Luna Experience-EC 400 lead to
decreasing the amount of PCEs. It is found that this decrease is statistically
significant (p<0.05).
References
- Gizem GÜLER, Cuma AKTAŞ, Ayla ÇELİK
- Mersin University, Graduate School of Natural and Applied Science, Mersin, Turkey Mersin University Faculty of Science and Letters, Department of Biology, Mersin, Turkey
Abstract
References
- Gizem GÜLER, Cuma AKTAŞ, Ayla ÇELİK
- Mersin University, Graduate School of Natural and Applied Science, Mersin, Turkey Mersin University Faculty of Science and Letters, Department of Biology, Mersin, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
