Abstract
The world’s
plant population, especially wild and endemic species of this population, is
unfortunately under extinction risk. For threatened population viability, plant
cryopreservation has a significant role to conserve genetic resources by in
vitro techniques, which involved in tissue culture, pre-growth, cryoprotection,
freezing (liquid nitrogen, -196 C), thawing, recovery (re-growth) and
regeneration. These steps are required for a successful cryopreservation but
genetic changes occur in plant tissue culture (somaclonal variation) and these
changes can cause the disruption of the genetic stability. There is an
increasing interest in investigation of genetic stability after
cryopreservation. In order to determine genetic changes between original
species and cryopreserved species, DNA-based techniques such as DNA-DNA
hybridisation and PCR techniques (randomly amplified polymorphic DNA (RAPD),
amplified fragment length polymorphism (AFLP), simple sequence repeat (SSR)
analysis) are useful, because they are simple, rapid and cost-effective, and
offer clear information.
AFLP method
allows the simultaneous analysis of many loci which widely spread over the
entire genome, without prior sequence knowledge of the organisms under study.
This is a very sensitive, reliable fingerprinting technique to resolve
differences between isolates of the same species in a broad range of taxa
including bacteria, animals, plants, and microalgae. For AFLP analysis
fluorescent markers, an automated sequencer and dedicated software are required
to detect polymorphic DNA fragments after DNA-primer amplification.
The monitoring of genetic stability after cryopreservation has a great
importance for plant genetic resources
References
- Mehmet Kadir ERDOĞAN
- Department of Biology, Faculty of Arts and Sciences, Bingöl University, 12000, Bingöl, Turkey
Abstract
References
- Mehmet Kadir ERDOĞAN
- Department of Biology, Faculty of Arts and Sciences, Bingöl University, 12000, Bingöl, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
