EVALUATION OF GENOTOXIC AND ANTIGENOTOXIC EFFECTS OF PUERARIN BY MICRONUCLEUS ASSAY ON MAMMALIAN CELL LINES
Abstract
It is well known
that free oxygen radicals play important role in the pathogenesis of chronic
disorders such as cancer, diabetes, cardiovascular and neurological diseases.
Recent interest in plant phenolics has increased because of their protective
effects against free oxygen radicals. Puerarin
[7-hydroxy-3-(4-hydroxyphenyl)-1-benzopyran-4-one 8-(β-D-glucopyranozid)],
isoflavone glycoside, primarily derived from Ge-gen (Pueraria lobata, Radix
Puerariae). This medicinal plant is commonly used in Chinese folk medicine.
Pueraria lobata has been used against alcohol dependency, hypertension, fever,
headache, diarrhea and eye disorders. Phytoestrogenic activity of puerarin due
to its isoflavone structure is very important in woman diseases. In the present
study genotoxic/antigenotoxic effects of puerarin were assessed by micronucleus
(MN) assay in human peripheral blood lymphocytes and Chinese hamster lung
fibroblast cell line (V79). In MN assay, the cells were treated with 1, 5, 10,
25 and 50 µM concentrations of puerarin. Hydrogen peroxide (H2O2), 50 µM, was
used as positive control and 1% DMSO was used as negative control. In our
study, the cells were treated with different concentrations of puerarin caused
no genotoxic effects alone at all studied concentrations as compared with the
negative control. MN frequencies of puerarin treated cells were found to be
decreased when compared to positive control. It seems that puerarin might have
a role in the prevention of genotoxic damage.
References
- Hatice Gül ANLAR, Merve BACANLI, A. Ahmet BAŞARAN, Nurşen BAŞARAN
- Hacettepe University Faculty of Pharmacy Department of Pharmaceutical Toxicology 06100 Ankara, Turkey Çukurova University Faculty of Pharmacy Department of Pharmaceutical Toxicology 06330 Adana, Turkey Hacettepe University Faculty of Pharmacy Department of Pharmacognosy 06100 Ankara, Turkey
Abstract
References
- Hatice Gül ANLAR, Merve BACANLI, A. Ahmet BAŞARAN, Nurşen BAŞARAN
- Hacettepe University Faculty of Pharmacy Department of Pharmaceutical Toxicology 06100 Ankara, Turkey Çukurova University Faculty of Pharmacy Department of Pharmaceutical Toxicology 06330 Adana, Turkey Hacettepe University Faculty of Pharmacy Department of Pharmacognosy 06100 Ankara, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
