COMBINATIONAL EFFECT OF ALYSSUM PATERI SUBSP. PROSTRATUM AND 5-FU ON HT-29 AND PC-3 CANCER CELL LINES
Abstract
Brassicaceae
(Cruciferae) family is widely distributed in the world with 350 genera and
about 3500 species. The species of this family have many health-promoting
phytochemicals such as minerals, fat, vitamins, phenolic compounds and soluble
sugars, and different species of Brassicaceae reduce the oxidative damage and
have anticarcinogenetic, antioxidant, antimicrobial, antiinflammatory effects.
A. pateri subsp. prostratum is a perennial member of Brassicaceae.
6,10,14-trimethyl-2-pentadecanone (22.09 %), 1,8-cineole (11.91 %),
thiobis-methane (6.43 %), 2,6,10-trimethylpentadecane (6.05 %) and hexadecane
(4.19 %) is the major constituents of the essential oil of A. pateri subsp.
prostratum.
The aim of this
study was examined the antiproliferative and apoptotic effect of hexane,
chloroform, ethyl acetate and methanol extracts from the aerial parts of
Alyssum pateri Nyár subsp. prostratum (Nyár) Dudley, which growing in Turkey,
and combination of these extracts with 5-FU. Cell Proliferation Kit I (MTT) and
Cell Death Detection Elisa Kit from Roche (Roche Diagnostics, Mannheim,
Germany) were used to determine the apoptotic and cytotoxic effect of Alyssum
pateri subsp. prostratum extracts on HT-29 human colorectal cancer and PC-3
human prostate cancer cell lines. Propidium iodide and ethidium bromide were
used for monitoring the apoptotic and necrotic cells.
References
- Mehmet Kadir ERDOĞAN1*, Lütfi BEHÇET
- Department of Biology, Faculty of Arts and Sciences, Bingöl University, 12000, Bingöl, Turkey
Abstract
References
- Mehmet Kadir ERDOĞAN1*, Lütfi BEHÇET
- Department of Biology, Faculty of Arts and Sciences, Bingöl University, 12000, Bingöl, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
