INVESTIGATION OF TOXICITY OF HYDROXYUREA ON DROSOPHILA MELANOGASTER (mwhxflr3)

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 140 - 140, 16.02.2017

Ayca Karasahınoglu Pınar Arslan Rabia Sarıkaya

Abstract

Hydroxyurea, a
cytostatic compound specific to the S phase of cell division, prevents
deoxyribonucleotide synthesis by inhibiting ribonucleotide reductase. In this
study, hydroxyurea toxicity, a retinoid and active ingredient of
antineoplastics, was investigated on Drosophila melanogaster (mwhxflr3).

72-h trans-heterozygous
larvae which are the mwhxflr3 Drosophila cross were chronically fed with 4 and
8 µg/mL concentrations of the hydroxyurea. Distilled water was used for the
control group. During the experiments, 3 g medium was wet with a 10 mL exposure
solution, and 100 larvae in each group were embedded in the medium. Adult flies
were counted after metamorphosis. Statistically significant differences in
percent survival between the experimental and control groups were compared by
using Chi-squared Test. The percentage of survival was 53 in 4 µg/mL
hydroxyurea, 58% in 8 µg/mL hydroxyurea. In the distilled water control group,
the percentage of survival was 97. There was a statistically significant
difference in favor of the control group between the survival percentage of 4
and 8 µg/mL hydroxyurea exposure groups and that of the control group
(p<0.001).

The results are
significant for reporting hydroxyurea to be toxic to Drosophila melanogaster
larvae. Investigation of hydroxyurea toxicity using different experimental
animals will be significant in terms of risk assessment.

Keywords

INVESTIGATION OF TOXICITY OF HYDROXYUREA ON DROSOPHILA MELANOGASTER (mwhxflr3)

References

• Ayca KARASAHINOGLU, Pınar ARSLAN1, Rabia SARIKAYA Department of Biology Education, Gazi Faculty of Education, Gazi University, Ankara Department of Primary Education, Gazi Faculty of Education, Gazi University, Ankara