Abstract
Investigations
the histogical effects of thiourea dioxide on gill tissue of zebrafish were
aimed.
Thioureadioxide
is an organosulfur compound which is a strong reducing agent. It is generally
used as decolorisation agent, bleach in textile industry. In the other
industrial fields such as leather processing, photographic, paper, pulp and
board industry it has a wide range of use. Industrial waste products have been
contaminated aquatic environment day after day.
After one week
adaptation period zebrafish divided into three group (n=10) as one control and
2 experimental groups (3mM, 4.5mM). For investigating the effects of thiourea
dioxide, intestine tissues were dissected after 5 day of the exposure. Tissues
were fixed with 10% neutral buffered formalin and dehydration was carried out
in an ascending series of ethanol. Tissues were cleared in xylene, embedded in
paraffin wax and cut into 5 µm sections on a microtome. The sections were
stained with hematoxylin (H&E). Results were evaluated with light
microscope.
In control group
normal gill histology has been observed. In 3 mM thiourea dioxide exposed
group, total fusion, dysmorphism and vacuolization at secondary lamellae,
hypertrophy at secretory cells, vacuolization and hyperplasia at primary
lamellae structure were detected. In 4.5 mM thiourea dioxide exposed group,
severe deterioration at secondary lamellae structure, hyperplasia and
vacuolization at primary lamellae and degeneration at secretory cells were
observed.
Keywords
HISTOLOGICAL EVALUATION OF THE EFFECTS OF THIOUREA DIOXIDE ON ZEBRAFISH (Danio rerio) GILLS
References
- Nazan Deniz YÖN, Zeynep İŞEL, Mustafa ZENGİN, Cansu AKBULUT
- Sakarya University Science and Letters Faculty Department of Biology, Sakarya, Tukey Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Tukey
Abstract
References
- Nazan Deniz YÖN, Zeynep İŞEL, Mustafa ZENGİN, Cansu AKBULUT
- Sakarya University Science and Letters Faculty Department of Biology, Sakarya, Tukey Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Tukey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
