HISTOLOGICAL EFFECTS OF TRIBENURON METHYL SPLEEN TISSUE OF ZEBRAFISH (Danio rerio)

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 152 - 152, 16.02.2017

Nazan Deniz Yön Burcu Öztürk Cansu Akbulut

Abstract

Examination the
histopathological effects of tribenuron methyl on spleen tissue of zebrafish were
aimed.

Tribenuron
methyl is an herbicide that used to control dicots in cereals and fallow land.
It inhibits the synthesis of aminoacids, specifically valine and isoleucine,
which prevents cell division and cell growth. It is generally applied as spray
and it access to soil directly and transferred to the roots. Pesticides access
aquatic ecosystems via ground waters and affect water quality and aquatic life.
In our study, examination the histopathological effects of tribenuron methyl on
spleen tissue of zebrafish were aimed.

Zebrafish were
raised in a computer-controlled incubation chamber, and received 14 hours of
daylight and 10 hours of darkness everyday. After one-week adaptation period
zebrafish divided into four group (n=10) as one control and 3 experimental
groups (40mg/L, 80mg/L, 120mg/L). For investigating the effects of tribenuron
methyl, spleen tissues were dissected after 5 day of the exposure. Tissues were
fixed with 10% neutral buffered formalin and dehydration were carried out in an
ascending series of ethanol. After tissues were cleared in xylene, embedded in
paraffin wax and cut into 5 µm sections on a microtome. The sections were
stained with hematoxylin(H&E). Results were evaluated with light
microscope.

In control group
normal spleen histology was observed. Normal splenic lymphoid tissue was
monitored. Melanin pigments and melanomacrophage cells were monitored clearly.

In 40 mg/L
exposure group, spleen sections have revealed that magnitude of tissue
alterations have sharply declined from severe degrees. Vacuolization were
observed milder forms. Disintegration of milder form were monitored.  Vacuolation without necrosis were detected.

In 80 mg/L
exposure group, increased amount of vacuolization and hyperplasia in the
melanomacrophage cells were observed at splenic tissue. Vacuolization were
observed melanin pigments. In 120 mg/L exposure group, necrosis was observed at
melanomacrophage center. Vacuolization was detected at melanomacrophage.
Histopathological lesions were observed in the splenic tissues and the spleens
appeared showing an increase in melanin pigments.

As a result, with this study, it is proved that tribenuron methyl cause
deterioration in fish spleen tissues.

Keywords

HISTOLOGICAL EFFECTS OF TRIBENURON METHYL SPLEEN TISSUE OF ZEBRAFISH (Danio rerio)

References

• Nazan Deniz YÖN, Burcu ÖZTÜRK, Cansu AKBULUT Sakarya University Science and Letters Faculty Department of Biology, Serdivan SAKARYA TURKEY