GENOTOXIC EFFECTS OF COBALT NANOPARTICLES AND IONIC FORM IN DROSOPHILA

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 165 - 165, 16.02.2017

Merve Güneş Burçin Yalçın Havva Ertuğrul Bülent Kaya

Abstract

Nanoparticles
widely used in different industrial areas because of their many advantages.
However, there has been little known about toxic effects of NPs on human and
environment. Today its known that most of diseases associated with mutation,
induced recombination and DNA damage. Therefore, determination of such effects
of NPs is important.

In this study,
the genotoxic effects of CoCl2 and CoNPs were investigated by using both
Somatic Mutation and Recombination Test (SMART) and Single Cell Gel
Electrophoresis (COMET) in Drosophila. SMART is reliable assay to detect a wide
range of genetic alterations in a rapid and inexpensive way. In SMART test, the
effects of these chemicals were evaluated according to genetic changes (point
mutation, deletion, non-disjunction and recombination) in wing imaginal disc
cells that lead to the formation of mutant trichomes. On the other hand, Alkaline
COMET assay is fast, powerfull and sensitive test to detect the single strand
breaks in alkali labile lesions individual cells in DNA.

CoCl2 showed the
positive results for small single spots, large single spot, total mwh spots and
total spots categories in only 10 mM concentration in trans-heterozygous flies
(mwh/flr3) while CoNP demonstrated positive results for same categories in 1
and 10 mM concentrations. Both CoCl2 and CoNP concentrations (0,1, 1 ve 10 mM)
significant induced the damage for tail moment and tail lenght.

Finally, used
chemicals which in this investigation observed in both COMET and SMART assay
cause genotoxic potential. 

Keywords

GENOTOXIC EFFECTS OF COBALT NANOPARTICLES AND IONIC FORM IN DROSOPHILA

References

• Merve GÜNEŞ, Burçin YALÇIN, Havva ERTUĞRUL, Bülent KAYA
• Akdeniz University, Faculty of Sciences, Biology Department, Antalya