ANTIMICROBIAL ACTIVITY, GENOTOXICITY AND DNA INTERACTIONS STUDIES OF SOME IMINE COMPOUNDS

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 170 - 170, 16.02.2017

Pınar Gök Neslihan Demır Mustafa Yıldız Bahar Gök

Abstract

Today, the
research and designing of new molecules which can interact with nucleic acids,
development of new anticancer drugs that can bind to DNA in chemotherapy is one
of the most studied topic. Although these genetic and cancer diseases are
caused by spontaneous mutations, the contribution of the surrounding external
chemical and physical agents is quite large. Therefore, studies on eliminatig
the effects of mutagenic subtances, preventing diseases and synthesis of the
novel antimutagenic agent which can be used intreatment of these diaseses has
been increased. Imine compounds have been found to be excellent inhibitors. We
have synthesized and characterized of the 3-aminopyridine Imine compounds. We
investigated of their antimicrobial activity, genotoxicity and DNA
interactions.

In this study,
Imine compounds synthesized from the reaction of
substitue-2-hydroxybenzaldehyde with 3-aminopyridine. We prepared solutions of
3-aminopyridine Imine compounds at 500, 50, 5 and 0.5 ppm concentrations. For
Ames test, Salmonella thyphimurium TA98 and TA100 mutant srains were used. The
interactions of the Schiff bases to calf tymus DNA (CT-DNA) and pBR322 DNA were
characterized by UV-vis spectroscopy and by agarose gel electrophoresis.
Escherichia coli, Pseudomonas aerouginosa, Proteus vulgaris, Staphylococcus
aureus, Enterococcus faecalis, Bacillus subtilis Candida albicans ve Candida
tropicalis were used as microorganisms for antimicrobial activity. Gentamicin,
ampicillin and fluconasol were used as controls in this study.

Consequently,
the Imine compounds have antimutagenic effects. The compounds have
intercalative binding. They were active against of yeasts and as well as active
against bacteries. 

Keywords

ANTIMICROBIAL ACTIVITY

References

• Pınar GÖK, Neslihan DEMIR, Mustafa YILDIZ, Bahar GÖK
• Çanakkale Onsekiz Mart University, Graduate School, Department of Biology 17100 Çanakkale Çanakkale Onsekiz Mart University, Faculty of Arts and Sciences, Department of Biology, 17100 Çanakkale Çanakkale Onsekiz Mart University, Faculty of Arts and Sciences, Department of Chemistry, 17100 Çanakkale