HISTOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) LIVER TISSUE
Abstract
Examination the
histopathological effects of (4s)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid on liver tissue of zebrafish were aimed.
(4S)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid is new synthesized substance which obtained from
cysteine and valine. Because of thiazolidine derivates have important
biological responses scientist work intensively on these compounds recent
years. It is obvious that thiazolidine contained compounds will be used in
future in the pharmaceutical industry to treat important diseases.
After one-week
adaptation period zebrafish divided into four group (n=10) as one control and 3
experimental groups (0,2 mM, 0,4 mM, 0,6 mM). For investigating the effects of
thiazolidine, liver tissues were dissected after 5 day of the exposure. Tissues
were fixed with 10% neutral buffered formalin and dehydration were carried out
in an ascending series of ethanol. After tissues were cleared in xylene,
embedded in paraffin wax and cut into 5 µm sections on a microtome. The
sections were stained with hematoxylin (H&E) and PAS. Results were
evaluated with light microscope.
In control group
normal liver histology was monitored. Parenchyma cells, hepatocytes and their
cytoplasm were observed clearly. Sinusiods and nuclei were easily monitored. In
the 0,2 mM exposure group, degeneration, contraction at vacuoles in hepatocyte
cytoplasm, hypertrophy. Degeneration was detected at hepatocyte cells. In 0,4
mM exposure group, some kupffer cells were observed in elliptical form.
Degeneration and vacuolization were observed on kupffer cells. In 0,6 mM
exposure group, vacuolization at vacuoles in hepatocyte cytoplasm were
monitored. Vacuolization and degeneration were also observed hepatocyte
cells. Vascular and sinusoidal
degeneration and steatozis were detected as well. As a result, with this study,
it is proved that (4s)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic
acid cause deterioration in fish liver tissues.
References
- Nazan Deniz YÖN, Burcu ÖZTÜRK, Mustafa ZENGİN, Cansu AKBULUT
- Sakarya University Science and Letters Faculty Department of Biology, SAKARYA TURKEY Sakarya University Science and Letters Faculty Department of Chemistry, SAKARYA TURKEY
Abstract
References
- Nazan Deniz YÖN, Burcu ÖZTÜRK, Mustafa ZENGİN, Cansu AKBULUT
- Sakarya University Science and Letters Faculty Department of Biology, SAKARYA TURKEY Sakarya University Science and Letters Faculty Department of Chemistry, SAKARYA TURKEY
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
