HISTOPATHOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) HEART TISSUE
Abstract
Examination the
histopathological effects of (4s)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic
acid on heart tissue of zebrafish were aimed.
Introduction:
(4S)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic acid is new
synthesized substance which obtained from cysteine and valine. Because of
thiazolidine derivates have important biological responses scientist work
intensively on these compounds recent years. It is obvious that thiazolidine
contained compounds will be used in future in the pharmaceutical industry to
treat important diseases.
After one-week
adaptation period zebrafish divided into four group (n=10) as one control and 3
experimental groups (0,2 mM, 0,4 mM, 0,6 mM). For investigating the effects of
thiazolidine, heart tissues were dissected after 5 day of the exposure. Tissues
were fixed with 10% neutral buffered formalin and dehydration were carried out
in an ascending series of ethanol. After tissues were cleared in xylene,
embedded in paraffin wax and cut into 5 µm sections on a microtome. The
sections were stained with hematoxylin(H&E) and PAS. Results were evaluated
with light microscope.
In control group
normal heart histology was observed. Bulb arteriosus, ventriculus, muscle cell
and endocardial cell were monitored clearly. In 0,2 mM exposure group,
degenerated muscle cells were detected. Hemorrhage at ventriculus, hyperplasia,
vacuolization and pleomorphic case at muscle cells, degeneration at pericardium
were observed. In 0,4 mM exposure group, hyperplasia at atrium endothelial
cells, vacuolization and degeneration at muscle cells were detected. In 0,6 mM
exposure group, leukocyte infiltration and pleomorphic case at ventriculus,
degeneration and vacuolization at pericardial cells, hyperplasia at bulbus
arteriosus were monitored.
References
- Nazan Deniz YÖN, Burcu ÖZTÜRK, Mustafa ZENGİN, Cansu AKBULUT
- Sakarya University Science and Letters Faculty Department of Biology, SAKARYA TURKEY Sakarya University Science and Letters Faculty Department of Chemistry, SAKARYA TURKEY
Abstract
References
- Nazan Deniz YÖN, Burcu ÖZTÜRK, Mustafa ZENGİN, Cansu AKBULUT
- Sakarya University Science and Letters Faculty Department of Biology, SAKARYA TURKEY Sakarya University Science and Letters Faculty Department of Chemistry, SAKARYA TURKEY
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
