Abstract
The use of herbal supplements (HSs) for treatment
erectile dysfunction (ED) has greatly increased in worldwide, mainly because of
a well-entrenched belief that herbal products are reliable and free from
adverse-toxic effects. Sildenafil (SD) is a potent and selective inhibitor of
cGMP specific PDE-5 and causes relaxation of smooth muscle, particularly in the
corpus cavernosum and pulmonary vasculature. The observed benefit of SD for the
treatment of ED has led to the increase in prevalence of illicit herbal
performance enhancement products. However, SD has serious side effects which
can be fatal. Due to increased use of SD in HSs and its serious side effects
have a non-sophisticated, simple, rapid, and reliable methods are needed. For
this purpose, we developed and validated HPLC-UV based analysis method for the
detection of SD in the HSs. Seperation was carried out by an octadecylsilane
C18 column. Mobile phase was consisted of acetonitrile, methanol and acetate
buffer (52:40:8, v/v/v). Flow was 1 mL/min. Total run was <14 min. UV was
set at 292 nm. Verapamil was used as an internal standard. A good accuracy
(bias <7.39) was achieved. Precision of the method was observed between
1.74-5.68 and 2.58-6.23 (RSD %) intra-day and inter-day, respectively.
Linearity, recovery, LOD-LOQ of the method has been determined. The method was
found to be linear, precise, accurate, and sensible. Method has been
successfully applied in analysis of 51 herbal products which were collected
from internet and the market. As a result, SD has been detected various
proportions in 18 products
References
- Emrah DURAL, Sinan SÜZEN, Tülin SÖYLEMEZOĞLU
- Cumhuriyet University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Sivas, Turkey Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey Ankara University, Institute of Forensic Sciences, Department of Forensic Toxicology, Ankara, Turkey
Abstract
References
- Emrah DURAL, Sinan SÜZEN, Tülin SÖYLEMEZOĞLU
- Cumhuriyet University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Sivas, Turkey Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey Ankara University, Institute of Forensic Sciences, Department of Forensic Toxicology, Ankara, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
