Abstract
In this study,
it was aimed that evaluate the hemolysis effect on ethanol assay.
In this study,
about 10 and 40 mg/dL concentration ethanol containing samples was prepared
with ethanol calibrator (92.7 mg/dL) and evaluated the effect of hemolysis. For
providing similar matrix, 10 ethanol-free normal patients’ serums were pooled
and used for diluations. Hemoglobin was isolated from fresh blood. Evaluation
was performed in 5 levels of hemolysis (about 0, 250, 500, 750, 1000 mg/dL).
It has been
shown to vary that about 10 mg/dL ethanol at 5 hemolysis levels 10.3, 10.5,
12.3, 14, 16.6 respectively. (According to the first reading percent
differences are 1.84, 19.42, 35.92, 61,17 respectively). It has been shown to
vary that about 10 mg/dL ethanol at 5 hemolysis levels 41.6, 41.6, 41.2, 43.9,
44.8 respectively (according to the first reading percent differences are 0,
-0,96, 5.53, 7.69 respectively).
Most of the
methods currently on the market use the same enzymatic reaction (alcohol
dehydrogenase). Therefore, a similar interference may occur in devices from
other brands. High rate of interference, especially in low level of ethanol,
may be associated with the analytical sensitivity.
Hemolysis
samples should be carefully considered to not experience legal troubles.
References
- Metin GENÇ, Özlem DOĞAN, Zihni KARAEREN, Aslıhan AVCI
- Ankara University, Faculty of Medicine, Department of Medical Biochemistry Ankara University, Ibni Sina Hospital, Central Laboratory
Abstract
References
- Metin GENÇ, Özlem DOĞAN, Zihni KARAEREN, Aslıhan AVCI
- Ankara University, Faculty of Medicine, Department of Medical Biochemistry Ankara University, Ibni Sina Hospital, Central Laboratory
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
