Increasing
pesticide usage will lead damage to the ecosystem and also human health. Allium
test is a rapid and sensitive assay to detect cytotoxicity of several
genotoxics and mutagenic agents. Cytotoxic effects of the herbicide Granweed
[%75 Tribenuron methyl] were investigated by using Allium test. Also
physiological effects (length of root and green leaf, chlorophy and MDA
contents) of Granweed were examined on Allium cepa L. The roots of the 4 days
old seedlings were treated with 0 g/lt (control=tap water), 0,05 g/lt, 0,1 g/lt,
0,2 g/lt and 0,4 g/lt concentrations of Granweed during 24, 48 and 72 hours.
Granweed significantly decreased Mitotic Index (MI) and increased the
chromosome aberrations (micronucleus, Anaphase bridge, sticky metaphase,
disturbed anaphase, c-metaphase) at all concentrations and treatment periods
when compared with their controls. It was also determined that Granweed
enhanced the Malondialdehide (MDA) level by increasing the lipid peroxidation,
decreased the length of root and green leaf and caused changes in chlorophyll
contents. All these changes were observed depending on concentration and
duration of applied herbicide.
Journal Section | Articles |
---|---|
Authors | |
Publication Date | February 16, 2017 |
Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |