ASSESSMENT OF THE GENOTOXICITY OF BUTYLPARABEN IN HUMAN LYMPHOCYTES USING THE COMET ASSAY AND CYTOKINESIS-BLOCK MICRONUCLEUS TEST

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 229 - 229, 16.02.2017

Gülsüm Handan Sınan Devrim Güzel Bayülken Berrin Ayaz Tüylü

Abstract

As a member of
the paraben family butylparaben is one of the most common preservative additive
in foods, pharmaceuticals and personal care products because of its
antimicrobial and antifungal activities. In this study, the genotoxicity of
butylparaben was investigated in human peripheral blood lymphocytes by using
cytokinesis-block micronucleus (CBMN) test and Comet assay. In addition, the
cytokinesis block proliferation index (CBPI) was measured for cytotoxicity.
Human lymphocytes were exposed to butylparaben different concentrations (10, 25,
50 and 100 µg/ml) for 24 and 48 hours at 37°C in CBMN test. For comet assay
freshly isolated human lymphocytes were exposed butlyparaben (10, 25, 50 and
125 µg/ml) for an hour. Decreased CBPI-values was observed at all dose
levels.  A dose and time dependent
increase of the micronucleated (MN) cell frequency was observed. In comet assay
butlyparaben increased the DNA migration in a dose-dependent manner. The
results demonstrated that butylparaben has genotoxic potential on human
lymphocytes.

 

Keywords

ASSESSMENT OF THE GENOTOXICITY OF BUTYLPARABEN IN HUMAN LYMPHOCYTES USING THE COMET ASSAY AND CYTOKINESIS-BLOCK MICRONUCLEUS TEST

References

• Gülsüm Handan SINAN, Devrim GÜZEL BAYÜLKEN, Berrin AYAZ TÜYLÜ
• Department of Biology, Faculty of Sciences, Anadolu University, Eskisehir, TÜRKIYE