DEVELOPMENT OF AN HPTLC METHOD FOR THE DETERMINATION OF PATULIN

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 241 - 241, 16.02.2017

Ömer Kurada Zeynep Türkmen Tarkan Barut Selda Mercan Münevver Açıkkol

Abstract

Mycotoxin
contamination in foods is one of the most important and unavoidable issue for
consumers and food industry. Patulin (PAT) is a toxic metabolite produced by
certain fungi species of Aspergillus, Penicillium, and Byssochlamys. It can be
found in many moldy fruits, vegetables, cereals and their puree. PAT is formed
and transferred via these food sources to human body due to the difficulties
experienced during the processing of food.

PAT is known as
mutagenic, immunotoxic, neurotoxic, and can cause adverse effects on the
gastrointestinal tract. Therefore, the amount of daily intake has a high
importance especially for infants and children. It is essential to protect
infants via controlling and monitoring levels of PAT in nutrition sources.
Codex Alimentarius and FDA set maximum allowable levels for concentrations of
PAT as "10 μg/kg in fruit juice or puree etc".

This study is
highlighting a High Performance Thin Layer Chromatography (HPTLC) method for
determination and quantitation of PAT in infant nutrition. Preliminary results
of HPTLC method will be presented.

Reference
standard of Patulin was diluted between 1-100 ng/µL for linearity study. Mobile
phase was chosen as Toluene: Ethyl acetate: Formic acid (5:4:1). Injection
volume was 1 µL. Injector washing cycle was set at three to prevent carry over.
Detection was done by 254 and 274 nm for comparison of the PAT under common and
specific wavelength. 

In this study,
linearity of PAT was evaluated with two different ranges; 1-10 ng/spot and
10-100 ng/spot. Correlation coefficients in linear regression mode were ≥0.997
and 0,986 with standard deviation 3,42% and 7,94% respectively. The Rf value
was found 0.55±0.04.   

After the
optimization and validation of this presented method, convenience foods for
infants such as fruit juice, puree will be extracted and analyzed to determine
PAT by this method, and daily intake limits will be calculated according to the
results. Thus, data obtained will show the possible presence of PAT as a
carcinogenic substance in infant food products.

Keywords

DEVELOPMENT OF AN HPTLC METHOD FOR THE DETERMINATION OF PATULIN

References

• Ömer KURADA, Zeynep TÜRKMEN, Tarkan BARUT, Selda MERCAN, Münevver AÇIKKOL
• Istanbul University, Institute of Forensic Sciences, 34089, Cerrahpasa, Istanbul, Turkiye