Abstract
Lipemic
specimens are common and frequent, but yet unresolved problem in clinical
chemistry, and may produce significant interferences in the analytical results
of different biochemical parameters. The aim of this study was to examine the
effect of lipid on ethanol assay.
In this study,
ethanol calibrator (92.7mg/dL) was used to prepare 10 and 40 mg/dL
concentration ethanol containing samples. 10 ethanol-free normal patients’
serum were pooled and used for providing similar matrix. Lipemic samples were
prepared by adding 5 levels (0, 250, 500, 750, 1000 mg/Dl) intralipid (%20)
(Baxter, Deerfield, IL).
The analytes
were then measured on Siemens Advia 2400 and the change of the analyte
concentrations was compared with baseline samples.
It has been
shown to vary that about 10 mg/dL ethanol at 5 lipemia levels 10.4, 10.7, 9.2, 9.3,
8,2 respectively. (according to the first reading percent differences are 3.88,
-11.6, -10.6, -21.2 respectively). It has been shown to vary that about 40
mg/dL ethanol at 5 lipemia levels 41.3, 41.9, 40.3, 39.9, 39.1 respectively
(according to the first reading percent differences are 1.45, -2,43, -3.4, -5.4
respectively).
Most
methodologies used on the oto-analyzer appeared to be subject to statistically
significant interference from lipemia when evaluated by standard statistical
methods, but these do not consider the analytical imprecision of assays. The
higher percentage change in the low concentration of ethanol, may be associated
with the analytical sensitivity.
This study has
useful information on ethanol interference by lipemia but it should be noted
that the in vitro results may not completely support what would happen in vivo.
Lipemic samples
should be carefully considered for medico-legal cases.
References
- Özlem DOĞAN, Metin GENÇ, Zihni KARAEREN, Berrin Imge ERGÜDER, Aslıhan AVCI
- Ankara University, Faculty of Medicine, Department of Medical Biochemistry Ankara University, Ibni Sina Hospital, Central Laboratory
Abstract
References
- Özlem DOĞAN, Metin GENÇ, Zihni KARAEREN, Berrin Imge ERGÜDER, Aslıhan AVCI
- Ankara University, Faculty of Medicine, Department of Medical Biochemistry Ankara University, Ibni Sina Hospital, Central Laboratory
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
