Abstract
Nowadays
according to the increased prevalence of metabolic disorders such as diabetes
and obesity, there is an increasing trend of consumption of non-caloric foods
and drinks. As a result of this situation there is a widely use of the extracts
of a natural plant, Stevia rebaudiana, or its compounds as a sweetener. In this
study, it was aimed to investigate possible toxixological effects of stevia and
its oxidative compounds on an oxidant parameter in liver tissue from rats.
For this aim, 26
adult Wistar type albino rat were used in the study. The animals were divided
into two groups as the control group (n=10) and the study group (n=16)
randomly. While the control group (Group 1) was fed only by standard rat diet,
the study group (Group 2) was fed by standard rat diet with a sweetener
including 22 mg stevia extract per day aurogastricly for one month. At the end
of the study, rats were sacrified and the liver tissues were surgically removed
to analyze an oxidant parameter which is Malondialdehyde-MDA levels. And also
histopathological examination and tissue DNA extraction were done.
According to the
results of the study in liver tissues, MDA levels of the study group which were
fed by stevia extract were found significantly higher than that in the control
group (p=0,021). The tissues of the group 2 displayed some histopathological
changes such as remarkable dilatation of sinusoids. There were no
histopathological changes in the structure of hepatocytes and also we did not
observe any apoptotic or necrotic cell and histopathological changes in the
portal area, except the cellular infiltration in a few focal portal areas.
There was no difference between groups according to DNA extraction findings.
According the
data of MDA levels and histopathological findings, it was thought that the
consumption of high dose of stevia as a sweetener may cause oxidant effects on
liver tissues.
References
- Ebru GÜRLEYİK, Tülin ÖZKAN, Sibel KILIÇOĞLU, Asuman SUNGUROĞLU, Aslıhan AVCI
- Council of Forensic Medicine, Ankara Ankara University, Faculty of Medicine, Ankara Ufuk University, Ankara
Abstract
References
- Ebru GÜRLEYİK, Tülin ÖZKAN, Sibel KILIÇOĞLU, Asuman SUNGUROĞLU, Aslıhan AVCI
- Council of Forensic Medicine, Ankara Ankara University, Faculty of Medicine, Ankara Ufuk University, Ankara
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
