ASSESSMENT OF INDIVIDUAL AND COMBINED CYTOTOXIC EFFECTS OF ZINC OXIDE AND COPPER OXIDE NANOPARTICLES IN L929 CELL CULTURES
Abstract
In the present
study, cytotoxic effects of zinc oxide (ZnO) and copper oxide (CuO)
nanoparticles (NPs) were determined by neutral red (NR) uptake assay following
exposure of L929 cell cultures to various concentrations of these NPs either
alone or as a mixture for 96 hours. Survival profiles of cell cultures were found
similar with ZnO and CuO NPs when the cells exposed to these NPs alone: The
decrease in cell survival were found dose-dependent. Nearly 50% decreases were
observed in the cell survival following exposure of cells to ≥ 5 μg/ml
concentrations of ZnO, and ≥ 0.5 μg/ml concentrations of CuO NPs. However, when
the cells exposed to mixture of these two NPs in various concentrations, ~50%
decrease in cell survival was noted at higher concentrations (≥ 100 μg/ml).
Diethylenetriaminepentaacetic acid (DTPA), N-acetyl cysteine (NAC) or taurine
(Tau) pretreatment of cell cultures were found to be marked protection against
cytotoxicity of 100 μg/ml ZnO and CuO NPs or mixtures of both NPs in 96 h
incubation. Reactive oxygen species (ROS) producing effects of these NPs were
also observed with their three different concentrations (100, 400, 800 μg/ml,
either alone or as a mixture) using a fluorescent probe
2′,7′-dichlorofluorescein-diacetate. The data obtained in this study suggest
that cytotoxic and ROS producing effects of these NPs are more pronounced when
they are used alone. Further studies are needed to determine underlying
mechanism of these effects.
This study was supported by Hacettepe
University Research Foundation (014 D12 301 001-830).
TEM images were obtained in Central
Laboratory of Middle East Technical University (METU) in Ankara, Turkey.
References
- Duygu PASLI, Aylin GÜRBAY
- Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey,
Abstract
References
- Duygu PASLI, Aylin GÜRBAY
- Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey,
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 16, 2017 |
| Published in Issue | Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |
