LIPID PEROXIDATION AND DNA DAMAGE IN PLASTIC WORKERS

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 289 - 289, 16.02.2017

Gülru Gürdemir Pınar Erkekoğlu , Ali Aşcı Kübra Gizem Yıldıztekin Aylin Balcı Ünzile Yaman Engin Tutkun Dinç Yılmaz Belma Gümüşel

Abstract

Phthalates are
added to polyvinyl (PVC) plastics in order to soften and give shape. PVC
plastics are usually used in plumbing pipes and guttering, shower curtains,
window frames, flooring and carpeting. Plastic industry offers services to a
range of industries, including aerospace, building, construction, home flooring
and carpeting products, electronics, packaging, and transportation. The main
aim of the present work is to determine the lipid peroxidation and DNA damage
in plastic workers (mean age: 30.71 ± 2.41 years, n=24), who were exposed to
phthalates [mainly di(2-ethylhexyl) phthalate] in their workplace. Control
group consisted of 29 men with a mean age of 35.86 ± 1.67 years. The mean
working period of the plastic workers was 8.17 years. Lipid peroxidation was
assessed by measuring urinary F2-isoprostane levels. DNA damage was evaluated
by measuring plasma 8-hydroxydeoxyguanosine (8-OHdG) levels. Urinary
F2-isoprostane levels of plastic workers (5.15 ± 0.52 ng/ml) were not
significantly higher than control (5.05 ± 0.52 ng/ml). However, plasma 8-OHdG
levels increased significantly in plastic workers (0.01 ± 0.002 ng/ml) vs.
control (0.14 ± 0.06 ng/ml). The urinary F2-isoprostane levels and plasma
8-OHdG levels were positively correlated and the correlation was statistically
significant in the plastic workers (r=0.413, p<0.05). Our results show that plastic
workers have high levels of DNA damage when compared to control, possibly due
to the oxidative damage caused by phthalate exposure. More studies are needed
to show the antioxidant/oxidant imbalance in plastic workers. 

Keywords

LIPID PEROXIDATION AND DNA DAMAGE IN PLASTIC WORKERS

References

• Gülru GÜRDEMİR, Pınar ERKEKOĞLU, Ali AŞCI, Kübra Gizem YILDIZTEKİN, Aylin BALCI, Ünzile YAMAN, Engin TUTKUN, Dinç YILMAZ, Belma GÜMÜŞEL
• Hacettepe University, Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey Atatürk University, Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey Erzincan University, Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey Ankara Occupational Diseases Hospital, Keçiören, Ankara, Turkey