QUANTITATIVE DETERMINATION OF SERUM CLOMIPRAMINE BY TANDEM MASS SPECTROMETRY

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 299 - 299, 16.02.2017

Abdullah Sıvrıkaya Ali Unlu , Sedat Abusoglu

Abstract

Therapeutic drug
monitoring of antidepressants and antipsychotics is necessary for an optimal
supervision of patients and their drug therapy to avoid medical complications,
intoxication, nonresponsiveness or noncompliance. The aim of this work was to
develop a simple, fast and accurate liquid-chromatography-mass spectrometry
method for determination and quantification of serum clomipramine.

For serum
clomipramine measurement, 200 µL of internal standard (acetaminophen solution)
was added to 200 µL serum in a glass tubes. Tubes were well mixed for a minute
and extracted twice with 3 ml of a solution of ethylacetate. After centrifuging
at 3000 rpm for 10 minutes, the organic phase was transferred to small glass
vials, dried completely under a steam of nitrogen at 400 ⁰C. Samples
were reconstituted with 200 µL methanol and 10 µL was injected and analyzed by
LC-MS/MS.

We developed an
analytical method for the simultaneous analysis of clomipramine in serum using
liquid chromatography-mass spectrometry (LC–MS/MS) with multiple selected ion
monitoring (SIM). The clomipramine assay was linear up to 200 µg/L.

Serum clomipramine
measurement can be easily performed by LC-MS/MS system to identify the risk of
the patients with therapeutic drug monitoring. This method allows accurate the
pathological levels with high linearity of the system.

This method
allows the exact determination of clomipramine levels in pathological
concentration to avoid toxic effects.

Keywords

QUANTITATIVE DETERMINATION OF SERUM CLOMIPRAMINE BY TANDEM MASS SPECTROMETRY

References

• Abdullah SIVRIKAYA, Ali UNLU, Sedat ABUSOGLU
• Department of Biochemistry, Selcuk University Faculty of Medicine, Konya, Turkey