Total Antioxidant Status and Hemocyte Counts of Crayfish (Astacus leptodacylus Eschscholtz 1823) after exposure to carbaryl and deltamethrin

Year 2017, Issue: Issue 1(2) - 3. INTERNATIONAL BIOCIDAL CONGRESS, 26 - 26, 01.02.2017

Aysel Çağlan Karasu Benli Duygu Şahin Burcu Koçak Memmi Aylin Sepici Dinçel

Abstract

Purpose:
Carbaryl (1-naphthyl methylcarbamate) and deltamethrin
[(S)α-cyano-3-phenoxybenzyl-(1R)-cis-3-(2.2-dibromovinyl)-2
2-dimethylcyclopropane carboxylate] are broad spectrum pesticides which are
widely used in field pest control, household use, and as veterinary and human
pediculicides and are among the most potent insecticides known. The narrow
clawed crayfish (Astacus leptodactylus Eschscholtz, 1823) was chosen as
representative of freshwater ecosystems, naturally distributed in water bodies
around Eurasia. This study presents the sublethal effects of these pesticides
on the total antioxidant status and hemocyte counts on narrow clawed crayfish.
Method: The narrow clawed crayfish (mean weight 32.78±7.12 g) were obtained
from local breeder in the pre-molt stage. After acclimatization period, they
were exposed to sublethal concentrations of two different pesticides for 2 and
7 days. Hemolymph samples were taken from the base of the second walking legs
via a 2.5 ml disposable syringe under ice anesthesia. Hemolymph total
antioxidant status (TAS) determinations were performed by a Randox commercial
kit (Randox Laboratories Ltd., Crumlin, UK). Total hemocyte counts were
performed by using hemocytometers. Findings: Total hemocyte counts were decreased
in both exposure groups when compared to control groups (P<0.05). Total
antioxidant status were significantly increased in carbaryl exposed groups
while did not altered in deltamethrin exposed groups. Result: The results in
this study demonstrate that total antioxidant status and hemocyte counts can
reliably be used as biomarkers to monitor pesticide in contaminated aquatic
life as early warning systems.

 

 

 

 

Keywords:
Total
Antioxidant Status, Total Hemocyte Counts, Crayfish (Astacus leptodacylus),
carbaryl, deltamethrin

Keywords

Total Antioxidant Status and Hemocyte Counts of Crayfish (Astacus leptodacylus Eschscholtz 1823) after exposure to carbaryl and deltamethrin

References

• Aysel Çağlan Karasu Benli, Duygu Şahin, Burcu Koçak Memmi, Aylin Sepici Dinçel Gazi Üniversitesi Ankara Başkent Üniversitesi Ankara Hacettepe Üniversitesi Ankara