Alternative Method Study for Determining Bactericidal Efficacy of Antimicrobial Paints

Year 2017, Issue: Issue 1(2) - 3. INTERNATIONAL BIOCIDAL CONGRESS, 29 - 29, 01.02.2017

Sennur Çalışkan Özdemir Ece Halat Aslı Şahiner

Abstract

Antimicrobial
paints contain various active substances to inhibit or reduce growth of microorganisms.
Interior and exterior wall paints with antimicrobial substances developed in
recent years started to be used in several areas with high infection risk
primarily in hospitals. In this study wall paints with 4 different active
substances from market were tested against S. aureus (ATCC 6538) and E.coli
(ATCC 10536) to determine and compare the bactericidal efficacy according to
ISO 22196. Within the scope of this study, cellulose filter papers were cut 5 x
5 cm of size and painted with antimicrobial paints. Paint without antimicrobial
substances were used as control for this test. Painted filter papers were left
to dry for one week. Painted filter papers and film coatings were exposed to
two sided UV sterilization. Suspension was prepared from 24 h bacterial
cultures with 105 cfu/ml concentration. 100 μl of bacterial suspensions were
inoculated on painted surfaces. After inoculation, surfaces were coated with
sterile parafilms. After 1, 3, 6 and 24 hour contact times in 37°C, filter
papers were transferred to bottles filled with SCDLP broth. Serial dilutions
were prepared and inoculations were made through pour plating method. Petri
dishes were incubated in 37°C for 24 h. Bactericidal efficacy of 4 different
active substances and 4 different contact times were determined. Paint with
0.10% zinc prythion provided 99.2% reduction against S.aureus at the 3rd hour,
all other active substances provided 99% reduction at 6th hour. Paint with 0.2
% AgCl provided 99.98% reduction against E.coli at the 3rd hour. In conclusion,
this study indicates that antibacterial efficacy of 4 antibacterial paints with
different active substances against E.coli and S.aureus start to increase after
6th hour. It was also observed that shorter contact time was effective with zinc
prythionon Gr (+), AgCl on Gr (-).

 

 

 

 

Keywords: Antimicrobial paint, bactericidal efficacy, Zinc prythion,
Silver chloride

Keywords

Alternative Method Study for Determining Bactericidal Efficacy of Antimicrobial Paints

References

• Sennur Çalışkan Özdemir, Ece Halat, Aslı Şahiner Ege University, Faculty of Science, Department of Biology, Izmir, Turkey